Different concentrations of interleukins in the peritoneal fluid of women with endometriosis: relationships with lymphocyte subsets
Different concentrations of interleukins in the peritoneal fluid of women with endometriosis: relationships with lymphocyte subsets
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DOI：
10.1080/09513590310001653044
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发表时间：
2004-03-01
影响因子：
2
通讯作者：
Volpe, A
中科院分区：
文献类型：
--
作者：
Gallinelli, A;Chiossi, G;Volpe, A
本研究探讨了子宫内膜异位症患者和非子宫内膜异位症患者腹腔液中IL-12或IL-13水平与免疫细胞亚群的可能关系。腹腔液样本取自80名因疼痛、不孕、输卵管结扎或再次吻合而接受腹腔镜检查的妇女。采用美国生育协会评分系统确定子宫内膜异位症的范围。腹腔液单个核细胞用细胞仪进行免疫表型分析，而腹腔液白细胞介素2浓度用两种超灵敏的商用酶联免疫吸附分析试剂盒测量。中、重度子宫内膜异位症(III、IV期)患者腹腔液IL-12水平显著高于健康对照组(P<0.01)。相反，子宫内膜异位症患者腹腔液IL-13浓度显著低于对照组，与疾病的严重程度无关(p<；0.05)。考虑免疫系统影响因素，子宫内膜异位症患者腹腔液CD8(+)/CD4(+)比值明显高于健康对照组。腹腔液CD8(+)和CD4(+)活化的T细胞数量前者明显低于后者，且与子宫内膜异位症分期无关。腹腔液T细胞与腹腔液IL-13水平呈负相关，腹腔液T细胞与IL-12水平呈正相关。这些数据似乎表明，子宫内膜异位症患者腹腔液细胞因子和T淋巴细胞亚群之间存在相互调节。
The present study explored the possible relationships between immune cell subsets and interleukin (IL)-12 or IL-13 levels in the peritoneal fluid of patients with and without endometriosis. Peritoneal fluid samples were obtained from 80 women while they were undergoing laparoscopy for pain, infertility, tubal ligation or re-anastomosis. The American Fertility Society scoring system was used to determine the extension of endometriosis. The peritoneal fluid mononuclear cells were analyzed for immunophenotyping using cytometry, whereas peritoneal fluid concentrations of interleukins were measured using two ultrasensitive commercially available enzyme-linked immunosorbent assay kits. Significantly higher peritoneal fluid IL-12 levels were found in women with moderate or severe endometriosis (stages III and IV) than in healthy controls (p < 0.01). Conversely, subjects with endometriosis showed remarkably lower peritoneal fluid IL-13 concentrations than controls, independent of the severity of the disease (p < 0.05). Considering immune system effectors, patients with endometriosis presented a significantly higher peritoneal fluid CD8(+)/CD4(+) ratio when compared with healthy controls. Moreover, the number of peritoneal fluid CD8(+) and CD4(+) activated T cells was significantly lower in the former than in the latter group, independent of the endometriosis stage. Connections were observed between peritoneal fluid interleukins and peritoneal fluid T cells: both patients with endometriosis and controls presented an inverse correlation between peritoneal fluid activated T cells and IL-13 levels, and a direct correlation between peritoneal fluid T cells and IL-12 concentrations. These data seem to suggest that a reciprocal modulation exists between peritoneal fluid cytokines and T lymphocyte subsets in patients with endometriosis.