Connectivity map of bipolar cells and photoreceptors in the mouse retina
Connectivity map of bipolar cells and photoreceptors in the mouse retina
复制标题
小鼠视网膜中双极细胞和光感受器的连接图
作者：
Christian Behrens;T. Schubert;S. Haverkamp;Thomas Euler;Philipp Berens
视觉处理从视觉系统的第一个突触开始。在小鼠视网膜中，三种不同类型的光感受器为 14 种双极细胞 (BC) 类型提供输入。传统上，大多数 BC 类型被认为接触其树突域内的所有锥体； ON BC 将仅通过所谓的内陷突触接触视锥细胞，而 OFF BC 将形成基底突触。通过挖掘公开的电子显微镜数据，我们发现了对外层视网膜连接规则的有趣违反：ON BC X 型仅接触了其树突区域中约 20% 的视锥细胞，并且大部分是非典型的非内陷接触。 5T、5O 和 8 型接触的视锥细胞也比预期少。此外，我们发现视杆细胞BC接收来自视锥细胞的输入，这提供了视杆细胞和视锥细胞通路在两个方向上相互连接的解剖学证据。这表明外丛状层的组织比传统认为的更复杂。
Visual processing begins at the first synapse of the visual system. In the mouse retina, three different types of photoreceptors provide input to 14 bipolar cell (BC) types. Classically, most BC types are thought to contact all cones within their dendritic field; ON BCs would contact cones exclusively via so-called invaginating synapses, while OFF BCs would form basal synapses. By mining publically available electron microscopy data, we discovered interesting violations of these rules of outer retinal connectivity: ON BC type X contacted only ~20% of the cones in its dendritic field and made mostly atypical non-invaginating contacts. Types 5T, 5O and 8 also contacted fewer cones than expected. In addition, we found that rod BCs received input from cones, providing anatomical evidence that rod and cone pathways are interconnected in both directions. This suggests that the organization of the outer plexiform layer is more complex than classically thought.