WEHI-13VAR - A STABLE AND SENSITIVE VARIANT OF WEHI-164 CLONE-13 FIBROSARCOMA FOR TUMOR-NECROSIS-FACTOR BIOASSAY
WEHI-13VAR - A STABLE AND SENSITIVE VARIANT OF WEHI-164 CLONE-13 FIBROSARCOMA FOR TUMOR-NECROSIS-FACTOR BIOASSAY
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DOI：
10.1016/0165-2478(95)00026-2
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发表时间：
1995-05-01
影响因子：
4.4
通讯作者：
ARMSTRONG, JA
中科院分区：
文献类型：
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作者：
KHABAR, KSA;SIDDIQUI, S;ARMSTRONG, JA
肿瘤坏死因子 (TNF) 的生物测定不仅依赖于对 TNF 作用敏感且稳定的细胞系。我们鉴定了 WEHI 164 克隆 13 的变体 WEHI-13VAR。当在放线菌素-D (AcD) 存在下进行测定时，该变体表现出对 rTNF-α 或 rTNF-β 的稳定性和高敏感性。从 WEHI-13VAR（rTNF-α；ED（50）范围：0.005-0.065 ng/ml）产生 15 个亚克隆。没有发现比 AcD 处理的 WEHI-13VAR 细胞系更敏感的克隆。该系列的一个重要特点是其稳定的灵敏度。在 AcD 存在的情况下，细胞系对 rTNF-β 敏感性的稳定性在 16 个月内得到证实，平均 ED(50) 为 0.046 +/- 0.004 ng/ml。总之，与其他敏感性丧失或稳定性未知的TNF敏感细胞系不同，WEHI-13VAR提供了一个灵敏、可靠和稳定的生物测定系统来检测细胞毒素、TNF-α和TNF-β。
Bioassaying tumor necrosis factor (TNF) relies not only on a sensitive but also on a stable cell line towards TNF action. A variant of WEHI 164 clone 13, WEHI-13VAR, was characterized by us. This variant demonstrated both stability and high sensitivity towards rTNF-alpha or rTNF-beta when assayed in the presence of actinomycin-D (AcD). Fifteen subclones were generated from WEHI-13VAR (rTNF-alpha; ED(50) range: 0.005-0.065 ng/ml). No clones were found more sensitive than the AcD-treated WEHI-13VAR cell line. An important feature of this line is its stable sensitivity. The stability of the cell line sensitivity towards rTNF-beta was demonstrated in the presence of AcD over a period of 16 months with an average ED(50) of 0.046 +/- 0.004 ng/ml. In conclusion, unlike other TNF-sensitive cell lines in which the sensitivity is lost or the stability is unknown, WEHI-13VAR provides a sensitive, reliable and stable bioassay system to detect cytotoxin, TNF-alpha, and TNF-beta.