Segregation distortion and linkage analysis in eggplant (Solanum melongena L.)
Segregation distortion and linkage analysis in eggplant (Solanum melongena L.)
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DOI：
10.1139/g10-073
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发表时间：
2010-10-01
期刊：
影响因子：
3.1
通讯作者：
Rotino, Giuseppe Leonardo
中科院分区：
文献类型：
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作者：
Barchi, Lorenzo;Lanteri, Sergio;Rotino, Giuseppe Leonardo
以茄子育种系305 E40和67/3的种内杂种为材料，构建了花药加倍单倍体（DH）群体和F2作图群体。前者采用了一个基因渗入片段从Solanum aethiopicum Gilo集团携带基因Rfo-sa 1，赋予抗尖孢镰刀菌;后者是一个选择从种内杂交涉及两个传统的茄子品种，缺乏Rfo-sa 1。利用28对AFLP引物组合对93个F-2和93个DH群体进行扩增，共扩增出170条多态性片段。在DH群体中，这些AFLP标记以及与Rfo-sa 1紧密连锁的标记中的117个标记的分离基本上是扭曲的，而在F-2群体中，分离扭曲仅限于10个标记，因此后者被选择用于图谱开发。一组141个F-2个体的基因型与73个AFLP PC（产生406个信息标记），32个SSR，4个番茄RFLP和3个CAPS标记连锁Rfo-sa 1。这导致348个标记分配到12个主要连锁群。该图谱覆盖了718.7 cM，包含238个标记（212个AFLP、22个SSR、1个RFLP和Rfo-sa 1 CAPS）。标记顺序和标记间的距离在这张茄子图谱在很大程度上是一致的，在最近发表的SSR图谱。从茄子育种的角度来看，花药培养产生的DH群体似乎受到大规模的分离失真，因此可能不是很有效地捕捉到的亲本系中存在的全方位的遗传变异。
An anther-derived doubled haploid (DH) population and an F-2 mapping population were developed from an intraspecific hybrid between the eggplant breeding lines 305E40 and 67/3. The former incorporates an introgressed segment from Solanum aethiopicum Gilo Group carrying the gene Rfo-sa1, which confers resistance to Fusarium oxysporum; the latter is a selection from an intraspecific cross involving two conventional eggplant varieties and lacks Rfo-sa1. Initially, 28 AFLP primer combinations (PCs) were applied to a sample of 93 F-2 individuals and 93 DH individuals, from which 170 polymorphic AFLP fragments were identified. In the DH population, the segregation of 117 of these AFLPs as well as markers closely linked to Rfo-sa1 was substantially distorted, while in the F-2 population, segregation distortion was restricted to just 10 markers, and thus the latter was chosen for map development. A set of 141 F-2 individuals was genotyped with 73 AFLP PCs (generating 406 informative markers), 32 SSRs, 4 tomato RFLPs, and 3 CAPS markers linked to Rfo-sa1. This resulted in the assignment of 348 markers to 12 major linkage groups. The framework map covered 718.7 cM, comprising 238 markers (212 AFLPs, 22 SSRs, 1 RFLP, and the Rfo-sa1 CAPS). Marker order and inter-marker distances in this eggplant map were largely consistent with those reported in a recently published SSR-based map. From an eggplant breeding perspective, DH populations produced by anther culture appear to be subject to massive segregation distortion and thus may not be very efficient in capturing the full range of genetic variation present in the parental lines.