Histone chaperone ASF1 mediates H3.3-H4 deposition in Arabidopsis.
Histone chaperone ASF1 mediates H3.3-H4 deposition in Arabidopsis.
复制标题
组蛋白伴侣 ASF1 介导拟南芥中 H3.3-H4 沉积
DOI：
10.1038/s41467-022-34648-0
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发表时间：
2022-11-15
影响因子：
16.6
通讯作者：
Jacobsen, Steven E.
中科院分区：
文献类型：
--
作者：
Zhong, Zhenhui;Wang, Yafei;Wang, Ming;Yang, Fan;Thomas, Quentin Angelo;Xue, Yan;Zhang, Yaxin;Liu, Wanlu;Jami-Alahmadi, Yasaman;Xu, Linhao;Feng, Suhua;Marquardt, Sebastian;Wohlschlegel, James A.;Ausin, Israel;Jacobsen, Steven E.
组蛋白伴侣和染色质重塑控制核小体动力学，这是转录，复制和DNA修复所必需的。组蛋白伴侣抗沉默因子1（ASF 1）在促进酵母和后生动物中CAF-1介导的复制依赖性H3.1沉积和HIRA介导的复制非依赖性H3.3沉积中起核心作用。ASF 1功能在植物中是否进化保守尚不清楚。在这里，我们表明，拟南芥ASF 1蛋白质显示的HIRA复杂的偏好。两个拟南芥ASF 1基因的同时突变引起的染色质密度和异位H3.1占有率通常富含H3.3的基因座的减少。遗传、转录组学和蛋白质组学数据表明，与CAF-1相比，ASF 1蛋白强烈偏好HIRA复合物。asf 1突变体也显示出虚假的Pol II转录起始的增加，并显示出基因体CG DNA甲基化的维持和组蛋白修饰的分布中的缺陷。此外，ASF 1的异位靶向导致过多的组蛋白沉积，染色质不易接近和基因沉默。这些发现揭示了ASF 1介导的组蛋白沉积对于基因组的适当表观遗传调节的重要性。组蛋白伴侣和染色质重塑控制核小体动力学。在这里，作者表明，拟南芥ASF 1分子伴侣主要提供组蛋白变体的HIRA复合物，以确保适当的表观遗传基因调控。
Histone chaperones and chromatin remodelers control nucleosome dynamics, which are essential for transcription, replication, and DNA repair. The histone chaperone Anti-Silencing Factor 1 (ASF1) plays a central role in facilitating CAF-1-mediated replication-dependent H3.1 deposition and HIRA-mediated replication-independent H3.3 deposition in yeast and metazoans. Whether ASF1 function is evolutionarily conserved in plants is unknown. Here, we show that Arabidopsis ASF1 proteins display a preference for the HIRA complex. Simultaneous mutation of both Arabidopsis ASF1 genes caused a decrease in chromatin density and ectopic H3.1 occupancy at loci typically enriched with H3.3. Genetic, transcriptomic, and proteomic data indicate that ASF1 proteins strongly prefers the HIRA complex over CAF-1. asf1 mutants also displayed an increase in spurious Pol II transcriptional initiation and showed defects in the maintenance of gene body CG DNA methylation and in the distribution of histone modifications. Furthermore, ectopic targeting of ASF1 caused excessive histone deposition, less accessible chromatin, and gene silencing. These findings reveal the importance of ASF1-mediated histone deposition for proper epigenetic regulation of the genome. Histone chaperones and chromatin remodelers control nucleosome dynamics. Here the authors show that the Arabidopsis ASF1 chaperone primarily supplies histone variants to the HIRA complex to ensure proper epigenetic gene regulation.
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影响因子：
64.5
作者：
Goldberg AD;Banaszynski LA;Noh KM;Lewis PW;Elsaesser SJ;Stadler S;Dewell S;Law M;Guo X;Li X;Wen D;Chapgier A;DeKelver RC;Miller JC;Lee YL;Boydston EA;Holmes MC;Gregory PD;Greally JM;Rafii S;Yang C;Scambler PJ;Garrick D;Gibbons RJ;Higgs DR;Cristea IM;Urnov FD;Zheng D;Allis CD
通讯作者：
Allis CD
影响因子：
11.6
作者：
Chen, Xiangsong;Lu, Li;Zhong, Xuehua
通讯作者：
Zhong, Xuehua
影响因子：
9.4
作者：
Benoit, Matthias;Simon, Lauriane;Probst, Aline V.
通讯作者：
Probst, Aline V.
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
14.9
作者：
Catoni M;Tsang JM;Greco AP;Zabet NR
通讯作者：
Zabet NR