Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae
Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae
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DOI：
10.1002/yea.1057
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发表时间：
2004-01-15
期刊：
影响因子：
2.6
通讯作者：
McCusker, JH
中科院分区：
文献类型：
--
作者：
Ito-Harashima, S;McCusker, JH
Novel MX cassettes are described that contain the open reading frames (ORFs) of Saccharomyces cerevisiae or Candida albicans LYS5. The LYS5MX and CaLYS5MX cassettes, the targeting efficiencies of which are equivalent. to those of other MX cassettes, are positively selected for Lys(+) in a lys5 background. Unlike most of the other MX cassettes, the LYS5MX cassettes are also negatively selectable (alpha-aminoadipate-resistant), which will allow the use of the LYS5MX cassettes in plasmid shuffling and will also greatly facilitate marker recycling. Copyright (C) 2003 John Wiley Sons, Ltd.
