High-throughput, temperature-controlled microchannel acoustophoresis device made with rapid prototyping
High-throughput, temperature-controlled microchannel acoustophoresis device made with rapid prototyping
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DOI：
10.1088/0960-1317/22/7/075017
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发表时间：
2012-07-01
影响因子：
2.3
通讯作者：
Bruus, Henrik
中科院分区：
文献类型：
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作者：
Adams, Jonathan D.;Ebbesen, Christian L.;Bruus, Henrik
我们报道了一种温控微流控声电泳装置，能够以大于1 L h（-1）的体积通量从未稀释的人全血中分离颗粒和转移血细胞。该装置是由玻璃基板和聚合物片在显微镜幻灯片格式使用低成本，快速原型技术。这种高通量声泳芯片（HTAC）利用温度稳定的驻波超声波，施加不同的声辐射力，可以根据尺寸、密度和可压缩性分离颗粒。该装置被证明能够分离10和2 μ m直径的聚苯乙烯珠的混合物，在1 L h（-1）的流速下分选效率为0.8。作为生物应用的第一步，HTAC还在处理人全血中进行了测试，并证明能够从未稀释的人全血中转移血细胞，在1 L h（-1）和2 L h（-1）时的效率分别为0.95和0.82。
We report a temperature-controlled microfluidic acoustophoresis device capable of separating particles and transferring blood cells from undiluted whole human blood at a volume throughput greater than 1 L h(-1). The device is fabricated from glass substrates and polymer sheets in microscope-slide format using low-cost, rapid-prototyping techniques. This high-throughput acoustophoresis chip (HTAC) utilizes a temperature-stabilized, standing ultrasonic wave, which imposes differential acoustic radiation forces that can separate particles according to size, density and compressibility. The device proved capable of separating a mixture of 10- and 2-mu m-diameter polystyrene beads with a sorting efficiency of 0.8 at a flow rate of 1 L h(-1). As a first step toward biological applications, the HTAC was also tested in processing whole human blood and proved capable of transferring blood cells from undiluted whole human blood with an efficiency of 0.95 at 1 L h(-1) and 0.82 at 2 L h(-1).