The Proliferative Status And Clonogenic Capacity Of Tumour Cells In A Transplantable Rhabdomyosarcoma Of The Rat Before And After Irradiation With 800 Rad Of X‐Rays
The Proliferative Status And Clonogenic Capacity Of Tumour Cells In A Transplantable Rhabdomyosarcoma Of The Rat Before And After Irradiation With 800 Rad Of X‐Rays
复制标题
大鼠移植性横纹肌肉瘤800 Rad X射线照射前后肿瘤细胞的增殖状态和克隆能力
DOI：
10.1111/j.1365-2184.1978.tb00877.x
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发表时间：
1978
影响因子：
8.5
通讯作者：
G. Barendsen
中科院分区：
文献类型：
--
作者：
A. F. Hermens;G. Barendsen
可移植到Wag/Rij大鼠近交系中的R-1横纹肌肉瘤用3 H-Tdr半连续标记，随后用X射线照射，以估计Q招募到P细胞中的速率，并进一步了解缺氧对克隆发生细胞的P和Q状态的重要性。通过在30小时内给予动物1次至总计7次3 H-Tdr腹膜内注射进行标记。在最后一次标记完成后45分钟，一半肿瘤接受800拉德300 Kv X射线的急性剂量，以将存活克隆发生细胞中氧合良好细胞的比例降低至低于1%;对标记的对照肿瘤进行假辐照。标记的肿瘤细胞的分数，LiTumour，从放射自显影中评价，所述放射自显影由照射后固定15分钟的肿瘤切片制备：标记的细胞灶的分数，LiFo，在从肿瘤的剩余部分分离的细胞的培养物制备的放射自显影中测定。
R‐1 Rhabdomyosarcomas Transplantable In The Inbred Strain Of Wag/Rij Rats Were Labelled Semicontinuously With 3H‐Tdr And Subsequently Irradiated With X‐Rays In Order To Estimate The Rate Of Recruitment Of Q Into P Cells And To Obtain More Insight Into The Importance Of Hypoxia With Respect To The P And Q Status Of Clonogenic Cells. Labelling Was Performed By Giving Animals From One To A Total Number Of Up To Seven Intraperitoneal Injections With 3H‐Tdr Within 30 Hr. Half Of The Number Of Tumours Received An Acute Dose Of 800 Rad Of 300 Kv X‐Rays At 45 Min After Completion Of The Last Labelling In Order To Reduce The Fraction Of Well Oxygenated Cells Among The Surviving Clonogenic Cells To Less Than 1%; Labelled Control Tumours Were Sham Irradiated. Fractions Of Labelled Tumour Cells, LiTumour’Were Evaluated From Autoradiographs Prepared From Sections Of Tumours That Were Fixed 15 Min After Irradiation: Fractions Of Labelled Cell Foci, LiFo, Were Determined In Autoradiographs Prepared From Cultures Of Cells Isolated From The Remaining Parts Of The Tumours.