Mechanosensing of substrate thickness.
Mechanosensing of substrate thickness.
复制标题
基材厚度的机械感应。
DOI：
10.1103/physreve.82.041918
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发表时间：
2010-10
期刊：
影响因子：
--
通讯作者：
Butler JP
中科院分区：
文献类型：
--
作者：
Lin YC;Tambe DT;Park CY;Wasserman MR;Trepat X;Krishnan R;Lenormand G;Fredberg JJ;Butler JP
贴壁细胞的结构和功能在很大程度上取决于其微环境，包括其基质的硬度。通常认为，基板厚度（与刚度相对）的作用可以忽略不计，因此可以认为是半无限的。这种说法最近受到质疑，但在这方面考虑的特征长度尺度知之甚少。我们在这里表明，这种特征长度尺度是横向细胞大小。随着基板厚度接近电池的横向尺寸，基板的表观刚度被放大到比基板的固有刚度大得多的水平。这种表观硬度的变化很容易被细胞感觉到，导致细胞伸展面积、硬度和收缩力的变化。相反，这些响应发生在细胞的长度，周围的孤立点力的mechanosensing的影响很大的内在基板刚度，但基板厚度可以忽略不计的程度。我们的结论是，基板厚度的机械传感在很大程度上由牵引力分布在横向尺寸的细胞占主导地位。
Structure and function of the adherent cell depend in a crucial way on its microenvironment, including the stiffness of its substrate. It is often asserted that substrate thickness (as opposed to stiffness) plays a negligible role and therefore may be considered semi-infinite. This assertion has been recently challenged, but the characteristic length scale to consider in this regard is poorly understood. We show here that this characteristic length scale is the lateral cell size. As substrate thickness approaches the lateral dimension of the cell, the apparent stiffness of the substrate is amplified to levels much greater than the intrinsic stiffness of the substrate. This change in apparent stiffness is readily sensed by the cell, leading to changes in cell spreading area, stiffness, and contractile forces. In contrast to these responses that occur over the length of the cell, mechanosensing around an isolated point force is influenced greatly by intrinsic substrate stiffness but to a negligible extent by substrate thickness. We conclude that mechanosensing of substrate thickness is dominated in large part by traction forces spread over the lateral dimension of the cell.