Tissue-engineering bone from omentum.
Tissue-engineering bone from omentum.
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DOI：
10.18999/nagjms.72.3-4.111
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发表时间：
2010-08
影响因子：
0.9
通讯作者：
Y. Kamei;K. Toriyama;T. Takada;Shunjiro Yagi
中科院分区：
文献类型：
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作者：
Y. Kamei;K. Toriyama;T. Takada;Shunjiro Yagi
骨组织工程是一个有趣的研究领域。近年来，骨组织工程的研究方法很多，如体外培养骨髓基质干细胞等。此外，还报道了血管化组织工程骨瓣的模型。然而，目前尚未见利用大网膜进行骨组织工程的报道。本文报道了一种从大网膜组织工程化骨的动物模型。在兔腹部，以胃网膜右血管为基础隆起的网膜被颅骨骨膜包裹。我们在手术后1、2、4、6、8、12或24周收获如此包裹的网膜。术后1周内，编织骨形成，成骨细胞成簇。在8周时，证实了髓化，包括粒细胞的存在。这项技术可能被证明是有用的创造组织工程骨瓣重建手术。
Tissue engineering of bone is an interesting field of research. Many approaches to bone tissue engineering such as from bone marrow stromal cells in vitro have been reported. Furthermore, a model of vascularized tissue-engineered bone flap has been reported. However, there has been no report of bone tissue engineering using omentum. We present a study of tissue engineering of bone from omentum in a rabbit model. The omentum, which was elevated based on the right gastroepiploic vessels, was wrapped by the periosteum from cranial bone in the abdomen of rabbits. We harvested the omentum thus wrapped 1, 2, 4, 6, 8, 12, or 24 weeks after surgery. Within 1 week after surgery, woven bone was formed and clusters of osteoblasts were observed. At 8 weeks, medullization, including the presence of granulocytes, was confirmed. This technique might prove useful for creating tissue-engineered bone flaps for reconstructive surgery.