Quantitation of Human Parvovirus B19 DNA in Erythema Infectiosum and Aplastic Crisis
Quantitation of Human Parvovirus B19 DNA in Erythema Infectiosum and Aplastic Crisis
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DOI：
10.1002/jmv.23930
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发表时间：
2014-12-01
影响因子：
12.7
通讯作者：
Tsutsumi, Hiroyuki
中科院分区：
文献类型：
--
作者：
Ishikawa, Aki;Yoto, Yuko;Tsutsumi, Hiroyuki
关于人细小病毒B19（B19 V）的实时PCR方法的一些出版物已经出现，一些病例报告提到了B19 V DNA载量。然而，没有大规模的研究定量水平的B19 V DNA在常见的或代表性的B19 V表现，如感染性红斑和再生障碍性危象已进行。因此，使用TaqMan PCR测定，定量了感染性红斑或再生障碍性危象受试者的大样本中的B19 V负荷。涉及感染性红斑急性期的65名受试者，此外还分析了来自7名患有B19 V相关再生障碍性危象并发慢性溶血性贫血的受试者的22份血清样本。在感染性红斑急性期，感染性红斑急性期血清样本中B19 V DNA载量中位数为7.63 × 10（5）个基因组/ml（范围为4.48 × 10（3）至8.31 × 10（6）个基因组/ml）。慢性溶血性贫血伴再生障碍性危象急性期血清B19 V DNA载量极高，为10（10）-10（13）个基因组/ml，1-2个月后逐渐下降至10（5）个基因组/ml左右。尽管所有受试者遵循几乎一致和典型的感染性红斑临床病程，但B19 V DNA载量存在较大的个体差异，即差异超过1,000倍。在再生障碍性危象受试者中观察到极高的B19 V负荷。这项研究是第一个大规模的研究报告的B19 V DNA载量的受试者感染性红斑和再生障碍性危象，最常见的和显着的临床表现由B19 V感染。医学病毒学杂志86：2102-2106，2014. (c)2014 Wiley Periodicals，Inc.
Several publications concerning the methods of real-time PCR for human parvovirus B19 (B19V) have appeared and some case reports mention B19V DNA loads. However, no large-scale study quantitating levels of B19V DNA in common or representative B19V manifestations such as erythema infectiosum and aplastic crisis has been performed. Consequently, using the TaqMan PCR assay, the B19V load in a large sample of subjects with erythema infectiosum or aplastic crisis was quantitated. Sixty-five subjects in the acute phase of erythema infectiosum were involved, and in addition 22 serum samples from seven subjects with B19V-associated aplastic crisis complicating chronic hemolytic anemia were also analyzed. In the acute phase of erythema infectiosum the median B19V DNA load in the serum samples from the acute phase of erythema infectiosum was 7.63x10(5)genomes/ml, (range from 4.48x10(3) to 8.31x10(6)genomes/ml). The serum B19V DNA load during the acute phase of aplastic crisis complicating chronic hemolytic anemia was extremely high, that is 10(10)-10(13)genomes/ml, and decreased gradually to around 10(5) genomes/ml over 1-2 months. Although all subjects followed an almost uniform and typical clinical course of erythema infectiosum, there was a large individual variation of B19V DNA loads, that is differences of over 1,000 times. Extremely high B19V loads were observed in subjects with aplastic crisis. This study is the first large scale report of studies of the B19V DNA loads in subjects with erythema infectiosum and aplastic crisis, the most common and significant clinical manifestations by B19V infections. J. Med. Virol. 86:2102-2106, 2014. (c) 2014 Wiley Periodicals, Inc.