Effects of CDKN2A (p16INK4A/p14ARF) Over-Expression on Proliferation and Migration of Human Melanoma A375 Cells (Retracted Article)
Effects of CDKN2A (p16INK4A/p14ARF) Over-Expression on Proliferation and Migration of Human Melanoma A375 Cells (Retracted Article)
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DOI：
10.1159/000453189
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发表时间：
2016-01-01
影响因子：
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通讯作者：
Wang, Xiao-Jun
中科院分区：
文献类型：
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作者：
Bai, Ming;Yu, Nan-Ze;Wang, Xiao-Jun
目的：探讨CDKN2A(p16(INK4A)/p14(ARF))过表达对人黑色素瘤A375细胞增殖和迁移的影响。方法：收集黑色素瘤组织和色素痣组织。将CDKN2A(p16(Ink4a))和CDKN2A(p14(ARF))高表达载体分别导入人黑色素瘤A375细胞，分为空白组、阴性对照(NC)组、p16(Ink4a)组和p14(ARF)组。用qRT-PCR和Western blotting检测CDKN2A(p16(Ink4a))和CDKN2A(p14(ARF))mRNA和蛋白的表达。采用CCK-8、流式细胞仪和Transwell法分别观察细胞增殖、细胞周期和细胞凋亡、迁移和侵袭情况。建立裸鼠皮下移植瘤模型，检测细胞在体内的生长情况。结果：与色素痣组织相比，黑色素瘤组织中CDKN2A(p16(Ink4a))和CDKN2A(p14(ARF))的mRNA和蛋白表达均显著降低。CDKN2A(p16(INK4A))和CDKN2A(p14(ARF))过表达抑制了A375细胞的增殖、迁移、侵袭和从G0/G1期向S期的进展，抑制了移植瘤的生长，但促进了细胞凋亡。结论：CDKN2A(p16(Ink4a))和CDKN2A(p14(ARF))过表达抑制了人黑色素瘤A375细胞的增殖和迁移。(C)2016年作者(S)，巴塞尔卡格尔股份公司出版
OBJECTIVE: This study aims to investigate the effects of CDKN2A (p16(INK4A)/p14(ARF)) over-expression on the proliferation and migration of human melanoma A375 cells. METHODS: Melanoma tissues and pigmented nevi tissues were collected. Human melanoma A375 cells were transfected by CDKN2A (p16(INK4A)) and CDKN2A (p14(ARF)) over-expressing vectors and then assigned into blank, negative control (NC), p16(INK4A) and p14(ARF) groups. The expression of CDKN2A (p16(INK4A)) and CDKN2A (p14(ARF)) mRNA and protein was detected by qRT-PCR and Western blotting. CCK-8, flow cytometry and Transwell assays were applied to observe cell proliferation, the cell cycle and apoptosis, and migration and invasion, respectively. The model of subcutaneous xenografts in nude mice was established to measure cell growth in vivo. RESULTS: Compared with pigmented nevi tissues, CDKN2A (p16(INK4A)) and CDKN2A (p14(ARF)) mRNA and protein expression were significantly decreased in melanoma tissues. CDKN2A (p16(INK4A)) and CDKN2A (p14(ARF)) over-expression inhibited proliferation, migration, invasion and progression from G0/G1 to S phase of A375 cells and xenograft tumor growth, but promoted apoptosis. CONCLUSION: Our study demonstrated that over-expression of CDKN2A (p16(INK4A)) and CDKN2A (p14(ARF)) suppressed proliferation and migration of human melanoma A375 cells. (C) 2016 The Author(s) Published by Karger AG, Basel