The combined effects of soya isoflavones and resistant starch on equol production and trabecular bone loss in ovariectomised mice.
The combined effects of soya isoflavones and resistant starch on equol production and trabecular bone loss in ovariectomised mice.
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DOI：
10.1017/s0007114516001537
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发表时间：
2016-07
期刊：
影响因子：
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通讯作者：
Y. Tousen;Y. Matsumoto;Chiho Matsumoto;Yoriko Nishide;Yuya Nagahata;Isao Kobayashi;Y. Ishimi
中科院分区：
文献类型：
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作者：
Y. Tousen;Y. Matsumoto;Chiho Matsumoto;Yoriko Nishide;Yuya Nagahata;Isao Kobayashi;Y. Ishimi
雌马酚是肠道微生物区系产生的大豆异黄酮类(ISO)大豆苷元的代谢物。与其他ISO相比，Equol具有更强的雌激素活性，它可以防止绝经后妇女的骨质流失。抗性淀粉(RS)具有益生菌活性，是一种膳食纤维，据报道可促进马儿的生产。相反，肠道微生物区系被报道通过减少骨骼中的炎性细胞因子水平和T淋巴细胞来直接调节骨骼健康。研究了饲料中添加ISO和RS对去卵巢(OVX)小鼠肠道微生物区系、马匹产量、骨密度(BMD)和骨髓炎症基因表达的影响。雌性ddy品系小鼠，8周龄，随机分为假手术组(Sham，n 7)和卵巢切除组(OVX)。OVX小鼠随机分为4组(每组7只)：OVX对照组(OVX)；OVX饲喂0.05%ISO饲料(OVX+ISO)；OVX饲喂9%RS饲料(OVX+RS)；OVX饲喂0.05%ISO+9%RS饲料(OVX+ISO+RS)。6周后，ISO和RS联合治疗增加了马匹产量，通过调节肠道环境，阻止了OVX诱导的股骨远端骨小梁BMD的下降，并改变了OVX诱导的骨髓炎症相关基因表达。然而，在去卵巢小鼠中，ISO+RS组和ISO单独组之间的骨参数没有显著差异。我们的研究结果表明，ISO和RS的结合可能改变了肠道微生物区系和骨髓中的免疫状态，导致去卵巢小鼠的骨吸收减弱。
Equol is a metabolite of the soya isoflavone (ISO) daidzein that is produced by intestinal microbiota. Equol has greater oestrogenic activity compared with other ISO, and it prevents bone loss in postmenopausal women. Resistant starch (RS), which has a prebiotic activity and is a dietary fibre, was reported to promote equol production. Conversely, the intestinal microbiota is reported to directly regulate bone health by reducing inflammatory cytokine levels and T-lymphocytes in bone. The present study evaluated the combined effects of diet supplemented with ISO and RS on intestinal microbiota, equol production, bone mineral density (BMD) and inflammatory gene expression in the bone marrow of ovariectomised (OVX) mice. Female ddY strain mice, aged 8 weeks, were either sham-operated (Sham, n 7) or OVX. OVX mice were randomly divided into the following four groups (seven per group): OVX control (OVX); OVX fed 0·05 % ISO diet (OVX+ISO); OVX fed 9 % RS diet (OVX+RS); and OVX fed 0·05 % ISO- and 9 % RS diet (OVX+ISO+RS). After 6 weeks, treatment with the combination of ISO and RS increased equol production, prevented the OVX-induced decline in trabecular BMD in the distal femur by modulating the enteric environment and altered OVX-induced inflammation-related gene expression in the bone marrow. However, there were no significant differences in bone parameters between the ISO+RS and ISO-alone groups in OVX mice. Our findings suggest that the combination of ISO and RS might alter intestinal microbiota and immune status in the bone marrow, resulting in attenuated bone resorption in OVX mice.