CHORIOGONADOTROPIN-LIKE ANTIGEN IN AN ANAEROBIC BACTERIUM, EUBACTERIUM-LENTUM, ISOLATED FROM A RECTAL TUMOR
CHORIOGONADOTROPIN-LIKE ANTIGEN IN AN ANAEROBIC BACTERIUM, EUBACTERIUM-LENTUM, ISOLATED FROM A RECTAL TUMOR
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DOI：
10.1128/iai.24.3.920-924.1979
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发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
CAMPBELLACEVEDO, EA
中科院分区：
文献类型：
--
作者：
ACEVEDO, HF;SLIFKIN, M;CAMPBELLACEVEDO, EA
用间接荧光素标记和间接过氧化物酶-抗过氧化物酶标记的免疫组织化学技术，并利用绒毛膜促性腺激素β-亚基的抗血清和总激素的抗血清，在一株最初从直肠肿瘤分离的乳杆菌中证明了绒毛膜促性腺激素样免疫反应物质的存在。相反，当应用于一株微小棒状杆菌以及致病和非致病的根癌农杆菌菌株时，这两种免疫组织化学反应均为阴性。这些结果首次证明了绒毛膜促性腺激素样抗原在专性厌氧菌中的表达，并支持了先前的发现，即绒毛膜促性腺激素样物质似乎只在癌症相关细菌中表达，但并不是所有与恶性肿瘤相关的细菌都有能力表达该抗原，至少在免疫组织化学检测到的数量上是如此。
Using the indirect fluorescein-labeled and indirect peroxidase-antiperoxidase-labeled immunohistochemical techniques, and utilizing both antiserum specific for the .beta.-subunit of choriogonadotropin and antiserum for the total hormone the presence of a choriogonadotropin-like immunoreactive material in a strain of E. lentum that was originally isolated from a rectal tumor was demonstrated. In contrast, both immunohistochemical reactions were negatiVe when applied to a strain of Corynebacterium parvum and to pathogenic and nonpathogenic strains of Agrobacterium tumefaciens. These results demonstrate for the 1st time the expression of the choriogonadotropin-like antigen in an obligate anaerobe and support previous findings that the choriogonadotropin-like material appears to be expressed only in cancer-associated bacteria, but that not all bacteria associated with the malignant neoplasms have the capacity to express the antigen, at least in amounts detectable by immunohistochemistry.