Photografting of 2-methacryloyloxyethyl phosphorylcholine from polydimethylsiloxane: Tunable protein repellency and lubrication property
Photografting of 2-methacryloyloxyethyl phosphorylcholine from polydimethylsiloxane: Tunable protein repellency and lubrication property
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DOI：
10.1016/j.colsurfb.2007.11.014
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发表时间：
2008-05-01
影响因子：
5.8
通讯作者：
Ishihara, Kazuhiko
中科院分区：
文献类型：
--
作者：
Goda, Tatsuro;Matsuno, Ryosuke;Ishihara, Kazuhiko
利用紫外线照射并使用二苯甲酮作为光引发剂，从聚二甲基硅氧烷（PDMS）基底上接枝聚合出磷酰胆碱基功能甲基丙烯酸酯单体2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）。研究了不同的单体浓度和照射时间，以验证特定生物条件下接枝密度和蛋白质抗性之间的关系。椭圆光度分析表明，接枝聚合物的层厚取决于照射 1 分钟后的单体浓度，但此后在所有指定条件下都稳定下来。 X射线光电子能谱分析得到的C Is谱曲线拟合表明，接枝聚合物的量随着单体浓度和照射时间的增加而增加。原子力显微镜图像显示，由于放大的链迁移性和随之而来的长度增长，接枝链之间的末端变得占主导地位。体外白蛋白和纤维蛋白原吸附结果表明，由于受控的移植物密度，可以通过特定条件轻松调节对蛋白质吸附的阻力。接枝显着增强了润滑性，并且良好溶剂中接枝密度的增加进一步促进了润滑性，表明接枝链与溶剂之间的相互作用形成了润滑系统。这些关于接枝 PDMS 表面的基本发现对于多种应用非常重要，包括将其用作生物材料和微流体装置。 (c) 2007 Elsevier B.V. 保留所有权利。
The phosphorylcholine group functional methacrylate monomer, 2-methacryloyloxyethyl phosphory1choline (MPC), was graft polymerized from the polydimethylsiloxane (PDMS) substrate using ultraviolet irradiation and using benzophenone as a photoinitiator. The varying monomer concentrations and irradiation times were investigated in order to verify the relationships between graft density and protein resistance under specific biological conditions. The ellipsometry analysis revealed that the layer thickness of the grafted polymer depended on the monomer concentrations after the irradiation for 1 min, however, it stabilized thereafter in all the specified conditions. The curve fitting of the C Is spectrum obtained by X-ray photoelectron spectroscopy analysis showed that the amount of grafted polymer increased with an increase in both monomer concentration and irradiation time. Atomic force microscopic images revealed that the terminations among the graft chains became dominant due to magnified chain mobility followed by growth of their length. In vitro albumin and fibrinogen adsorption results indicated that the resistance to protein adsorption was easily tuned by the specified conditions due to the controlled graft density. Lubrication was dramatically enhanced by the grafting and it was further promoted by an increase in the graft density in good solvents, indicating that the interactions between the graft chains and the solvents resulted in the lubrication system. These basic findings regarding the grafted PDMS surface are important for versatile applications, including its use as a biomaterial and microfluidic device. (c) 2007 Elsevier B.V. All rights reserved.