A digital microfluidic platform for the automation of quantitative biomolecular assays
A digital microfluidic platform for the automation of quantitative biomolecular assays
复制标题
DOI：
10.1039/b920124f
复制
发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Mathies, Richard A.
中科院分区：
文献类型：
--
作者：
Jensen, Erik C.;Bhat, Bharath P.;Mathies, Richard A.
开发并优化了用于定量、多步骤生物分子测定自动化的数字微流体平台。该平台由一个2维微阀阵列组成，可以对微阀进行编程，以使用纳升规模体积的样品执行试剂路由、混合、冲洗、连续稀释和许多其他操作。微阀之间流体的离散转移的特征在于使用重量流量分析和优化，以实现最大效率。优化了片上试剂混合和系列稀释方案，以在1000倍稀释范围内实现线性。这些优化的程序用于开发一种快速，定量测定过氧化氢，氧化应激的生物标志物。一个亚微摩尔的检测限证明了8.5分钟的程序运行时间，从而建立了这个平台作为一个有效的工具，多步生物测定的自动化。该系统的可编程性使得能够在通用芯片格式上快速开发各种检测方案。
A digital microfluidic platform for the automation of quantitative, multi-step biomolecular assays is developed and optimized. The platform consists of a 2-dimensional array of microvalves that can be programmed to perform reagent routing, mixing, rinsing, serial dilution, and many other operations using nanolitre scale volumes of sample. Discrete transfer of fluid between microvalves is characterized using gravimetric flow analysis and optimized to achieve maximum efficiency. Protocols for on-chip reagent mixing and serial dilution are optimized to achieve linearity over a 1000-fold dilution range. These optimized programs are used to develop a rapid, quantitative assay for hydrogen peroxide, a biomarker of oxidative stress. A sub-micromolar limit of detection is demonstrated with an 8.5 min program runtime, thus establishing this platform as an effective tool for the automation of multi-step bioassays. The programmability of this system enables rapid development of diverse assay protocols on a common chip format.