Integration of immunoassay into extended nanospace
Integration of immunoassay into extended nanospace
复制标题
将免疫测定整合到扩展的纳米空间中
DOI：
10.1007/s00604-008-0075-5
复制
发表时间：
2009
影响因子：
5.7
通讯作者：
T. Kitamori
中科院分区：
文献类型：
--
作者：
R. Kojima;K. Mawatari;Björn Renberg;Takehiko Tsukahara;T. Kitamori
我们报告了首次成功地将夹心免疫分析小型化到用于检测甲胎蛋白的扩展纳米空间通道。为了便于液体处理，采用光刻、电子束曝光、电感耦合等离子体和热键合技术，构建了一种具有纳米通道和两个微通道的熔融二氧化硅微芯片。试剂可以被选择性地引入纳米通道，并通过使用交替打开和关闭进出口的压力控制器来阻止流动。介绍了用于免疫分析的纳米微芯片装置的结构和使用方法。
We report on the first successful miniaturization of sandwich immunoassay into an extended nanospace channel for the detection of alpha-fetoprotein. A fused silica microchip with a nanochannel flanked by two microchannels, to facilitate liquid handling, was constructed using photo- and electron beam lithography, ICP and thermal bonding. Reagents could be selectively introduced into the nanochannel and the flow be stopped by using a pressure controller that alternatively opened and closed inlet and outlet ports. The construction and handling of the nano-in-microchip device for immunoassay is described.