Probing gender-specific lipid metabolites and diagnostic biomarkers for lung cancer using Fourier transform ion cyclotron resonance mass spectrometry
Probing gender-specific lipid metabolites and diagnostic biomarkers for lung cancer using Fourier transform ion cyclotron resonance mass spectrometry
复制标题
使用傅里叶变换离子回旋共振质谱法探索肺癌的性别特异性脂质代谢物和诊断生物标志物
DOI：
10.1016/j.cca.2012.08.010
复制
发表时间：
2012-12-24
影响因子：
5
通讯作者：
Li, Zhili
中科院分区：
文献类型：
--
作者：
Guo, Yumei;Wang, Xianmin;Li, Zhili
背景：目前尚无有效的临床生物标志物用于肺癌（LC）的早期和特异性检测。LC患者部分血清代谢物水平的变化与患者性别和LC分期有关。方法：采用直接输注正离子电喷雾电离-傅里叶变换离子回旋共振质谱法结合单变量和偏最小二乘判别分析（PLS-DA）对LC患者（58例）和健康对照（495例）的血清代谢物进行测定。结果：单因素分析表明，与健康对照相比，LC患者212种血清代谢物中有141种发生了显著变化。基于141种代谢物的PLS-DA模型显示了单性别LC患者与相应健康对照组之间代谢物分布的差异，以及LC女性与LC男性之间代谢物分布的差异。几种脂质包括脂肪酸衍生物，鞘磷脂（SM）和溶血磷脂酰胆碱（LPC）与LC进展有关。以油酰胺、长链酰基肉毒碱、LPC（18:1）、LPC（20:4）、LPC（20:3）、LPC（22:6）和SM（16:0/1）作为生物标志物进行分类，LC患者与健康对照有明显的分离，敏感性和特异性分别为100%和91%。结论：本研究发现的血清代谢物可能在性别特异性LC检测和癌症早期诊断中发挥重要作用。(C) 2012 Elsevier B.V.版权所有
Background: There are no effective clinical biomarkers for early and specific detection of lung cancer (LC). The changes in the levels of some serum metabolites of LC patients are associated with patient gender and LC stages.Methods: Serum metabolites of the LC patients (n = 58) and healthy controls (n = 495) were performed using direct-infusion positive ion electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry in combination with univariate and partial least squares discriminant analyses (PLS-DA).Results: Univariate analysis indicated that 141 of the 212 serum metabolites were significantly changed in the LC patients compared with healthy controls. PLS-DA model, based on the 141 metabolites, demonstrated the differential metabolite distribution in single-sex LC patients compared with the corresponding healthy controls, and also in LC females compared with LC males. Several lipids comprising fatty acid derivations, sphingomyelin (SM) and lysophosphatidylcholine (LPC) were associated with the LC progression. Classification using oleamide, long chain acyl carnitines, LPC(18:1), LPC(20:4), LPC(20:3), LPC(22:6), and SM(16:0/1) as a biomarker panel resulted in remarkable separation between the LC patients and healthy controls with sensitivity and specificity of 100% and 91%, respectively.Conclusion: The serum metabolites found in this study may play essential roles in gender-specific LC detection and early diagnosis of cancer. (C) 2012 Elsevier B.V. All rights reserved.