Functional microdomains of glycolipids with partially fluorinated membrane anchors: impact on cell adhesion.
Functional microdomains of glycolipids with partially fluorinated membrane anchors: impact on cell adhesion.
复制标题
具有部分氟化膜锚的糖脂的功能微域：对细胞粘附的影响。
作者：
C. Gege;Matthias F. Schneider;G. Schumacher;L. Limozin;U. Rothe;G. Bendas;Motomu Tanaka;R. Schmidt
通过将新糖脂与部分氟烷基(F-烷基)链和基质脂与烷基链混合来设计糖脂的功能微域。气-水界面混合脂单分子膜的荧光图像表明，通过烷基和F-烷基锚定的强烈分离可以控制微区的大小和分布，而碳水化合物头部基团似乎起到了相当小的作用。这些单层中的微区可以转移到疏水的衬底上，并在动态流动室中进行实验。这些结果清楚地表明，表达E-选择素的中国仓鼠卵巢细胞(CHO-E细胞)在含有唾液酸路易斯X微域(SLeX)的脂单分子层上的动态黏附可以通过控制配体和基质的分离来增强和降低。此外，在巨大的脂泡中也可以形成相同的SLeX簇，这可以作为局部表达生物特异性功能的模型细胞。
Functional microdomains of glycolipids were designed by mixing neoglycolipids with partially fluorinated alkyl (F-alkyl) chains and matrix lipids with alkyl chains. Fluorescence images of the mixed lipid monolayers at the air-water interface demonstrated that it is possible to control both size and distribution of the microdomains by means of the strong demixing of alkyl and F-alkyl membrane anchors, while the carbohydrate head groups seemed to play a rather minor role. These microdomains in monolayers could be transferred onto hydrophobized substrates and subjected to experiments in a dynamic flow chamber. The results obtained here clearly indicated that the dynamic adhesion of Chinese hamster ovarial cells expressing E-selectin (CHO-E cells) on a lipid monolayer containing microdomains of sialyl Lewisx (sLex) can be both enhanced and reduced by controlled demixing of ligands and matrices. Moreover, the same clusters of sLex could also be formed in giant lipid vesicles, which can be used as a model cell that locally expresses biospecific functions.