STEPWISE INTRACLONAL MATURATION OF ANTIBODY-AFFINITY THROUGH SOMATIC HYPERMUTATION
STEPWISE INTRACLONAL MATURATION OF ANTIBODY-AFFINITY THROUGH SOMATIC HYPERMUTATION
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DOI：
10.1073/pnas.85.21.8206
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发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
RAJEWSKY, K
中科院分区：
文献类型：
--
作者：
KOCKS, C;RAJEWSKY, K
使用重组DNA技术，我们重建了一个系谱树(Sablitzky，F.，Wildner，G.和Rajeski，K.(1985)EMBO J.4,345-350)，它将产生体细胞突变抗体的三个克隆相关的B细胞与表达生殖系编码抗体的祖细胞连接起来。体细胞突变体是从体内的免疫反应中分离出来的。胚系编码的祖细胞抗体与抗原结合亲和力高。无性系内亲和力成熟是在15倍范围内逐步发生的。
Using recombinant DNA techniques, we reconstructed a genealogical tree (Sablitzky, F., Wildner, G. and Rajewsky, K. (1985) EMBO J. 4, 345-350) that connects three clonally related B cells producing somatically mutated antibodies to a progenitor cell expressing a germ line-encoded antibody. The somatic mutants had been isolated from an in vivo immune response. The germ line-encoded progenitor antibody bound the antigen with high affinity. Intraclonal affinity maturation occurred stepwise over a 15-fold range.