Detection, synthesis, structure, and function of oligo(3-hydroxyalkanoates): contributions by synthetic organic chemists
Detection, synthesis, structure, and function of oligo(3-hydroxyalkanoates): contributions by synthetic organic chemists
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DOI：
10.1016/s0141-8130(99)00037-9
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发表时间：
1999-06-01
影响因子：
8.2
通讯作者：
Fritz, MG
中科院分区：
文献类型：
--
作者：
Seebach, D;Fritz, MG
已经确定了两种类型的生物大分子聚（R-3-羟基烷酸酯）：高分子量微生物储存材料（sPHA）和短链品种，其由丁酸酯和戊酸酯残基组成，与其他生物大分子如聚磷酸钙或蛋白质（cPHB/PHV）复合。虽然sPHA已经吸引了世界各地众多科学家的关注，但对cPHB和结构和功能相关的聚苹果酸酯（PMA）的研究仍处于起步阶段。本文综述了单分散性cPHAs、寡聚化cPHAs、聚羟基聚乙烯醇（PVP）、聚甲基丙烯酸甲酯（PVP）的化学合成、结构、功能及相互作用（3-羟基烷酸酯），重点是丁酸酯（OHB）;我们报道了迄今未发表的关于cPHB和PMA的酶促降解，关于生物样品中HB、HV检测的新分析方法，和OHB介导的Ca 2+运输通过磷脂双分子层的人工囊泡，最后，我们讨论了可能的机制，通过细胞膜的离子运输，所造成的cPHB。推测性和连续性的问题是问是否结构简单的PHAs可能已经演变为存储材料和两亲性大分子之前的多肽，β-淀粉酶，和核酸，在生命的历史，或在益生元条件下。(C)1999 Elsevier Science B. V.保留所有权利。
Two types of the biological macromolecules poly(R-3-hydroalkanoates) have been identified: the high-molecular-weight microbial storage material (sPHA) and a short-chain variety, consisting of butyrate and valerate residues, complexed with other biomacromolecules such as calcium polyphosphate or proteins (cPHB/PHV). While sPHA has attracted, and still enjoys, a lot of attention from numerous scientists around the world, research on cPHB and the structurally and functionally related polymalate (PMA) is still in its infancy. In this article, we present a review on the chemical synthesis, structure, function and interactions of monodisperse cPHAs, the oligo( 3-hydrosyalkanoates), with emphasis on the butyrates (OHB); we report hitherto unpublished results on the enzymatic degradation of cPHB and PMA, on a new analytical method for HB,HV detection in biological samples, and on OHB-mediated Ca2+ transport through phospholipid bilayers of artificial vesicles; finally, we discuss possible mechanisms of ion transport through cell membranes, as caused by cPHB. The speculative-and provocative-question is asked whether the structurally simple PHAs may have evolved as storage materials and amphiphilic macromolecules before poly-peptides, -saccharides, and -nucleic acids, in the history of life, or under prebiotic conditions. (C) 1999 Elsevier Science B.V. All rights reserved.