COMPOSITE IS1 ELEMENTS ENCODING HYDROXAMATE-MEDIATED IRON UPTAKE IN FIME PLASMIDS FROM EPIDEMIC SALMONELLA-SPP
COMPOSITE IS1 ELEMENTS ENCODING HYDROXAMATE-MEDIATED IRON UPTAKE IN FIME PLASMIDS FROM EPIDEMIC SALMONELLA-SPP
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DOI：
10.1128/jb.162.1.307-316.1985
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
MAIMONE, F
中科院分区：
文献类型：
--
作者：
COLONNA, B;NICOLETTI, M;MAIMONE, F
11种Fime质粒代表在S.wien和S.对北非、欧洲和中东分离的鼠伤寒沙门氏菌进行了检查，以确定其是否存在致炎性、粘附性和铁螯合机制的决定因素。化学和遗传数据表明，所有的质粒编码异羟肟酸介导的铁同化系统。FIME质粒pZM 61的衍生质粒和克隆片段的详细分析表明，含有异羟肟酸生物合成和泄殖腔素DF 13受体基因的DNA区域的一般遗传和结构组织与pColV-K30的气杆菌素介导的Fe摄取系统所述的基本相同。这个DNA区域是一个16.7个碱基长的复合元件的一部分，并携带其IS 1模块作为反向重复序列。在分析的所有9种FIME质粒中，在任一方向上都存在非常相似的元件。
Eleven FIme plasmids representative of those identified in epidemic strains of S.wien and S. typhimurium isolated in North Africa, Europe, and the Middle East were examined for the presence of determinants of toxigenicity, adherence and Fe-sequestering mechanisms. Chemical and genetic data indicated that all plasmids code for a hydroxamate-mediated Fe assimilation system. Detailed analysis of derivative plasmids and cloned fragments of FIme plasmid pZM61 demonstrated that the general genetic and structural organization of the DNA region containing the genes for hydroxamate biosynthesis and cloacin DF13 receptor was virtually identical to that described for the aerobactin-mediated Fe uptake system of pColV-K30. This DNA region is part of a composite element that is 16.7 kilobases long and carries its IS1 modules as inverted repeats. A very similar element is present in either orientation in all 9 FIme plasmids analyzed.