Immunoferritin determination of the distribution of (Na+ + K+) ATPase over the plasma membranes of renal convoluted tubules. II. Proximal segment.
Immunoferritin determination of the distribution of (Na+ + K+) ATPase over the plasma membranes of renal convoluted tubules. II. Proximal segment.
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DOI：
10.1083/jcb.68.2.304
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发表时间：
1976-02
期刊：
影响因子：
--
通讯作者：
Kyte J
中科院分区：
文献类型：
--
作者：
Kyte J
(Na+ + K+) ATP酶在犬肾皮质近曲小管质膜上的分布已被确定。该组织的超薄冰冻切片用针对该酶的兔抗体和铁蛋白缀合的山羊抗兔丙种球蛋白染色。事实证明，高浓度的这种酶均匀地排列在该上皮的细胞间隙中。这一观察结果的结果是根据这些小管的低阻力紧密连接和它们支持的等渗流体运输来讨论的。此外，(Na+ + K+) ATP酶抗体可识别刷状缘内小管管腔表面上的抗原。据推测，该酶也存在于质膜的该区域中，尽管浓度低得多。为了进一步证实这一结论，从兔肾中纯化了刷状缘部分，并显示其含有显着的 (Na+ + K+) ATP 酶。这些结果与先前关于 (Na+ + K+) ATP 酶在该组织中的位置的结论相矛盾。
The distribution of (Na+ + K+) ATPase over the plasma membranes of the proximal convoluted tubule from canine renal cortex has been determined. Ultrathin frozen sections of this tissue were stained with rabbit antibodies to this enzyme and ferritin-conjugated goat antirabbit gamma-globulin. It is demonstrated that high concentrations of this enzyme uniformly line the intercellular spaces of this epithelium. The consequences of this observation are discussed in terms of the low resistant tight junctions of these tubules and the isotonic fluid transport which they support. Furthermore, antibodies to (Na+ + K+) ATPase recognize an antigen on the luminal surfaces of the tubules within the brush border. It is proposed that the enzyme is present in this region of the plasma membrane as well, although at much lower concentration. To further substantiate this conclusion, a brush border fraction has been purified from rabbit kidney and been shown to contain significant (Na+ + K+) ATPase. These results contradict earlier conclusions about the location of (Na+ + K+) ATPase in this tissue.