Monoclonal antibodies to stromal cell types of the mouse thymus
Monoclonal antibodies to stromal cell types of the mouse thymus
复制标题
针对小鼠胸腺基质细胞类型的单克隆抗体
DOI：
10.1002/eji.1830140608
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发表时间：
1984
影响因子：
5.4
通讯作者：
W. van Ewijk
中科院分区：
文献类型：
--
作者：
E. van Vliet;M. Melis;W. van Ewijk
已经制备了七种分泌针对小鼠胸腺非淋巴细胞的单克隆抗体（mAb）的杂交瘤细胞系。这些单克隆抗体清楚地证明了胸腺基质的异质性。根据免疫过氧化物酶技术在冷冻组织切片上观察到的解剖分布模式，将它们分为四组。第一组 mAb，ER-TR1、2 和 3，检测由主要组织相容性复合物的 I 区编码的抗原。这些抗原在淋巴器官的基质细胞和淋巴样细胞上表达。第二类单克隆抗体 ER-TR4 与胸腺皮质的上皮细胞发生反应。第三组的单克隆抗体检测胸腺髓质的基质细胞。该组中的一种抗体 ER-TR5 专门与髓质上皮细胞发生反应。 ER-TR6 是该组的另一种抗体，与髓质叉指细胞和巨噬细胞发生反应。第四种抗体 ER-TR7 可检测胸腺的网状成纤维细胞。讨论了本抗体检测到的胸腺细胞类型在 T 细胞分化中的可能作用。
Seven hybridoma cell lines secreting monoclonal antibodies (mAb) to nonlymphoid cells of the mouse thymus have been prepared. These mAb clearly demonstrate the heterogeneity of the thymic stroma. Based on their anatomical distribution patterns observed with the immunoperoxidase technique on frozen tissue sections, they were subdivided into four groups. The first group of mAb, ER‐TR1, 2 and 3, detects antigens encoded for by the I region of the major histocompatibility complex. These antigens are expressed on both stromal and lymphoid cells in lymphoid organs. mAb of the second category, ER‐TR4, react with epithelial cells in the thymic cortex. mAb of the third group detect stromal cells of the thymic medulla. One antibody of this group, ER‐TR5, exclusively reacts with medullary epithelial cells. ER‐TR6, the other antibody of this group, reacts with medullary interdigitating cells and macrophages. The fourth type of antibodies, ER‐TR7, detects the reticular fibroblasts of the thymus. The possible role of the thymic cell types detected by the present antibodies in T cell differentiation is discussed.