Transposition of plasmid-borne Tn10 elements does not exhibit simple length-dependence.
Transposition of plasmid-borne Tn10 elements does not exhibit simple length-dependence.
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DOI：
10.1093/genetics/111.4.705
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发表时间：
1985-12
期刊：
影响因子：
3.3
通讯作者：
J. Way;Nancy Kleckner
中科院分区：
文献类型：
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作者：
J. Way;Nancy Kleckner
转座子长度每增加一个酶，转座子从细菌染色体到F表位组的Tn10元件的转座频率降低40%。这种关系的基础尚不清楚。我们现在已经研究了小的多拷贝质粒的缺陷Tn10元件的互补转座。我们发现，在这种情况下的长度依赖性是减少或不存在，这取决于具体类别的转座事件。这些观察结果可以被解释为对模型的证据，染色体长度依赖性的发生，因为衰变的转座相关的复制复合物。这种解释与表明Tn10转座通常是非复制性的无关实验一致。长度依赖现象的替代解释进行了讨论。
The transposition frequencies of Tn10 elements from the bacterial chromosome to an F epitome decrease 40% for every kilobase increase in transposon length. The basis for this relationship is not known. We have now examined complemented transposition of defective Tn10 elements off small multicopy plasmids. We find that length dependence in this situation is either reduced or absent, depending on the specific class of transposition events involved. These observations can be interpreted as evidence against the model that chromosomal length dependence occurs because of decay of a transposition-associated replicative complex. This interpretation is consistent with unrelated experiments suggesting that Tn10 transposition is normally nonreplicative. Alternative explanations of length dependence phenomena are discussed.