Taxonomy of Australian clinical isolates of the genus Photorhabdus and proposal of Photorhabdus asymbiotica subsp asymbiotica subsp nov and P-asymbiotica subsp australis subsp nov.
Taxonomy of Australian clinical isolates of the genus Photorhabdus and proposal of Photorhabdus asymbiotica subsp asymbiotica subsp nov and P-asymbiotica subsp australis subsp nov.
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DOI：
10.1099/ijs.0.03005-0
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发表时间：
2004-07-01
影响因子：
2.8
通讯作者：
Beard, CE
中科院分区：
文献类型：
--
作者：
Akhurst, RJ;Boemare, NE;Beard, CE
对从澳大利亚人临床标本中培养的Photorhabdus分离株与从美国人临床标本中分离的Photorhabdus共生菌以及与昆虫病原线虫共生的Photorhabdus属物种的关系进行了评估。采用DNA-DNA杂交、16S rRNA和gyrB基因序列的系统发育分析和表型分析的多相方法。这些研究表明，gyrB基因序列数据与DNA-DNA杂交数据和表型数据具有很好的相关性，但16S rRNA基因序列数据不适用于光毛藻属的物种划分。澳大利亚临床分离株与美国临床分离株的亲缘关系最近，但两组之间存在明显差异。一新亚种--非生物光轮藻亚种。南方亚种。11月(模式菌株9802892(T)=CIP 108025(T)=ACM5210(T))。非生物亚种。11月对gyrB序列的分析，结合先前发表的DNA-DNA杂交和16S rRNA基因的PCR-RFLP分析数据，表明已描述的发光光头藻亚种超过三个亚种，并证实了先前提出的光头藻亚种划分的有效性。虽然在gyrB系统发育分析中，一株不发光的共生分离物与非共生性假单胞菌聚在一起，但DNA-DNA杂交表明该分离物不属于非共生性假单胞菌，共生种和临床种之间存在明显的区别。
The relationship of Photorhabdus isolates that were cultured from human clinical specimens in Australia to Photorhabdus asymbiotica isolates from human clinical specimens in the USA and to species of the genus Photorhabdus that are associated symbiotically with entomopathogenic nematodes was evaluated. A polyphasic approach that involved DNA-DNA hybridization, phylogenetic analyses of 16S rRNA and gyrB gene sequences and phenotypic characterization was adopted. These investigations showed that gyrB gene sequence data correlated well with DNA-DNA hybridization and phenotypic data, but that 16S rRNA gene sequence data were not suitable for defining species within the genus Photorhabdus. Australian clinical isolates proved to be related most closely to clinical isolates from the USA, but the two groups were distinct. A novel subspecies, Photorhabdus asymbiotica subsp. australis subsp. nov. (type strain, 9802892(T)=CIP 108025(T)=ACM 5210(T)), is proposed, with the concomitant creation of Photorhabdus asymbiotica subsp. asymbiotica subsp. nov. Analysis of gyrB sequences, coupled with previously published data on DNA-DNA hybridization and PCR-RFLP analysis of the 16S rRNA gene, indicated that there are more than the three subspecies of Photorhabdus luminescens that have been described and confirmed the validity of the previously proposed subdivision of Photorhabdus temperata. Although a non-luminescent, symbiotic isolate clustered consistently with P. asymbiotica in gyrB phylogenetic analyses, DNA-DNA hybridization indicated that this isolate does not belong to the species P. asymbiotica and that there is a clear distinction between symbiotic and clinical species of Photorhabdus.