Genetic characterization of highly pathogenic avian influenza H5N1 viruses isolated from naturally infected pigeons in Egypt
Genetic characterization of highly pathogenic avian influenza H5N1 viruses isolated from naturally infected pigeons in Egypt
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DOI：
10.1007/s11262-016-1369-z
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发表时间：
2016-12-01
期刊：
影响因子：
1.6
通讯作者：
Nakaya, Takaaki
中科院分区：
文献类型：
--
作者：
Elgendy, Emad Mohamed;Watanabe, Yohei;Nakaya, Takaaki
禽流感病毒不仅在家禽中而且在人类中造成严重的死亡率和发病率，从而给公共卫生造成严重负担。虽然家禽对禽流感病毒感染的易感性已得到很好的表征，但鸽子被认为对这些病毒的易感性较低。然而，最近的研究报告，自然鸽子感染高致病性禽流感H5 N1病毒。埃及是H5 N1禽流感流行地区之一，在农场后院和屋顶上建造的塔中饲养鸽子，这为鸽子向人类传播病毒提供了潜在风险。在这项研究中，我们进行了遗传分析的两个H5 N1病毒株，从自然感染的鸽子在埃及。遗传和系统发育分析表明，这些病毒源自在鸡或鸭中传播的埃及H5 N1病毒。在内部基因中检测到了几种独特的突变，以前在任何埃及分离株中都没有报道过（即，聚合酶残基PB 1-V3 D、PB 1-K363 R、PA-A369 V和PA-V602 I;核蛋白残基NP-R38 K;和非结构蛋白残基NS 1-D120 N和NS 2-F55 C）。我们的研究结果表明，鸽子自然感染H5 N1病毒，可以成为一个潜在的水库，传播给人类，并显示了遗传分析的重要性，H5 N1内部基因。
Avian influenza viruses impose serious public health burdens with significant mortality and morbidity not only in poultry but also in humans. While poultry susceptibility to avian influenza virus infection is well characterized, pigeons have been thought to have low susceptibility to these viruses. However, recent studies reported natural pigeon infections with highly pathogenic avian influenza H5N1 viruses. In Egypt, which is one of the H5N1 endemic areas for birds, pigeons are raised in towers built on farms in backyards and on house roofs, providing a potential risk for virus transmission from pigeons to humans. In this study, we performed genetic analysis of two H5N1 virus strains that were isolated from naturally infected pigeons in Egypt. Genetic and phylogenetic analyses showed that these viruses originated from Egyptian H5N1 viruses that were circulating in chickens or ducks. Several unique mutations, not reported before in any Egyptian isolates, were detected in the internal genes (i.e., polymerase residues PB1-V3D, PB1-K363R, PA-A369V, and PA-V602I; nucleoprotein residue NP-R38K; and nonstructural protein residues NS1-D120N and NS2-F55C). Our findings suggested that pigeons are naturally infected with H5N1 virus and can be a potential reservoir for transmission to humans, and showed the importance of genetic analysis of H5N1 internal genes.