ZNF307, a novel zinc finger gene suppresses p53 and p21 pathway
ZNF307, a novel zinc finger gene suppresses p53 and p21 pathway
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ZNF307，一种新型锌手指基因，抑制 p53 和 p21 通路
DOI：
10.1016/j.bbrc.2007.08.180
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发表时间：
2007-11-30
影响因子：
3.1
通讯作者：
Wu, Xlushan
中科院分区：
文献类型：
--
作者：
Li, Jing;Wang, Yuequn;Wu, Xlushan
我们克隆了一个新的KRAB相关的锌指基因ZNF 307，编码545个氨基酸的蛋白质。ZNF 307在物种进化中是保守的，并且在人类成人和胎儿组织中差异表达。EGFP-ZNF 307融合蛋白定位于细胞核。转录活性测定显示ZNF 307抑制L 8 G5-荧光素酶的转录活性。ZNF 307在不同细胞系中的过表达也抑制了p53和p21的转录活性。此外，ZNF 307通过降低p53蛋白水平起作用，而p53蛋白的降低是通过增加MDM 2和EP 300的转录来实现的。ZNF 307可能通过激活MDM 2和EP 300的表达，诱导p53降解，从而抑制p53-p21通路。(C)2007年爱思唯尔公司All rights reserved.
We have cloned a novel KRAB-related, zinc finger gene, ZNF307, encoding a protein of 545 aa. ZNF307 is conserved across species in evolution and is differentially expressed in human adult and fetal tissues. The fusion protein of EGFP-ZNF307 localizes in the nucleus. Transcriptional activity assays show ZNF307 suppresses transcriptional activity of L8G5-luciferase. Overexpressing ZNF307 in different cell lines also inhibits the transcriptional activities of p53 and p21. Moreover, ZNF307 works by reducing the p53 protein level and p53 protein reduction is achieved by increasing transcription of MDM2 and EP300. ZNF307 might suppress p53-p21 pathway through activating MDM2 and EP300 expression and inducing p53 degradation. (C) 2007 Elsevier Inc. All rights reserved.