MicroRNA-34a attenuates the proliferation, invasion and metastasis of gastric cancer cells via downregulation of MET
MicroRNA-34a attenuates the proliferation, invasion and metastasis of gastric cancer cells via downregulation of MET
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DOI：
10.3892/mmr.2015.4110
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发表时间：
2015-10-01
影响因子：
3.4
通讯作者：
Zhong, Xiao Gang
中科院分区：
文献类型：
--
作者：
Wei, Bin;Huang, Qiao Ying;Zhong, Xiao Gang
增殖、浸润和转移是胃癌的主要特征，导致全球胃癌患者的高死亡率。作为p53的直接靶点，microRNA（miR）-34a在胃癌发生发展中的作用是重要的，但存在争议。在本研究中，研究了GC标本（n=40）中miR-34 a的临床重要性，并在癌症基因组图谱（TCGA; n=352）的独立队列中得到证实。在TCGA队列中使用Kaplan-Meier生存曲线结合完整的随访数据分析miR-34 a的预后价值（n=157）。检测miR-34 a在人胃癌细胞系和正常胃上皮细胞系中的表达水平。使用细胞计数试剂盒8、集落形成和细胞侵袭测定来评估miR-34 a对增殖和侵袭的影响。通过生物信息学预测确定miR-34 a的分子基础。使用逆转录-定量聚合酶链反应和蛋白质印迹分析评估miR-34 a与MET之间的相关性。结果表明，胃癌组织中miR-34 a表达较正常胃组织明显下调（P
Proliferation, invasion and metastasis are key features of gastric cancer, contributing to high mortality rates in patients with gastric cancer worldwide. As a direct target of p53, the functions of microRNA (miR)-34a are important, but controversial, in the progression of gastric cancer. In the present study, the clinical importance of miR-34a in GC specimens (n=40) were investigated and were confirmed in an independent cohort from The Cancer Genome Atlas (TCGA; n=352). The prognostic value of miR-34a was analyzed using a Kaplan-Meier survival curve in the TCGA cohort, in combination with complete follow-up data (n=157). The level of miR-34a was detected in the human gastric cancer cell line and normal gastric epithelial cell line. The effect of miR-34a on proliferation and invasion were evaluated using Cell Counting Kit 8, colony formation and cell invasion assays. The molecular basis of miR-34a was determined by bioinformatics prediction. The correlation between miR-34a and MET was assessed using reverse transcription-quantitative polymerase chain reaction and western blot analyses. The results indicated that miR-34a was downregulated in the gastric cancer tissues, compared with the normal gastric tissues (P