Dual roles of hydrogen peroxide in promoting zebrafish renal repair and regeneration
Dual roles of hydrogen peroxide in promoting zebrafish renal repair and regeneration
复制标题
过氧化氢促进斑马鱼肾脏修复和再生的双重作用
DOI：
10.1016/j.bbrc.2019.06.052
复制
发表时间：
2019-08-27
影响因子：
3.1
通讯作者：
Liu, Chi
中科院分区：
文献类型：
--
作者：
Chen, Jianli;Yu, Ting;Liu, Chi
急性肾损伤（阿基）是一种在数小时或数天内发生的严重肾功能衰竭或肾损伤。目前，阿基尚无获批的药物治疗。斑马鱼是研究阿基修复的极好模型，因为它具有显著的修复肾损伤的能力。利用庆大霉素诱导的斑马鱼阿基模型，我们发现过氧化氢（H2 O2）在阿基的恢复过程中起着双重作用，包括肾脏修复和肾脏再生。在阿基的修复阶段，H2 O2在近端和远端肾小管产生。通过使用Duox Vivo-Morpholino或化学抑制剂抑制H2 O2产生，观察到肾小管严重损伤和广泛的细胞凋亡。在再生阶段，我们发现H_2O_2在肾组织中大量产生。抑制H_2O_2的产生可显著下调肾再生能力，这与肾祖细胞增殖障碍有关。因此，H2 O2是斑马鱼肾损伤修复的保护因子，也是肾脏再生的起始信号，提示H2 O2在促进斑马鱼阿基恢复中具有重要作用。(C)2019爱思唯尔公司All rights reserved.
Acute renal injury (AKI) is a serious disorder of renal failure or renal damage that occurs within hours or days. At present, there is no approved pharmaceutical treatment for AKI. Zebrafish is an excellent model for studying the repair of AKI because of its remarkable ability to repair kidney injury. Using zebrafish AKI model inducing by gentamicin, we found that hydrogen peroxide (H2O2) plays dual roles during the period of AKI recovery including renal repair and kidney regeneration. In the repair stage of AKI, H2O2 was produced in proximal and distal segments of renal tubules. By inhibiting H2O2 generation using Duox Vivo-Morpholino or chemical inhibitor, it was observed of severe damage of renal tubules, and extensive cell apoptosis. In the stage of regeneration, we found that H2O2 was highly generated in renal interstitium. Inhibiting production of H2O2 could significantly down-regulate the ability of kidney regeneration, which was associated with the failure of proliferation of renal progenitor cells. Therefore, H2O2 acts as a protective factor in renal repair and an initial signal of kidney regeneration, indicating the key roles of H2O2 in promoting recovery of AKI in zebrafish. (C) 2019 Elsevier Inc. All rights reserved.