Virulence factor profiles and antimicrobial susceptibilities of ocular Bacillus isolates
Virulence factor profiles and antimicrobial susceptibilities of ocular Bacillus isolates
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DOI：
10.1080/02713680600850963
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发表时间：
2006-09-01
影响因子：
2
通讯作者：
Stroman, David W.
中科院分区：
文献类型：
--
作者：
Callegan, Michelle C.;Cochran, Daniel C.;Stroman, David W.
芽孢杆菌引起最迅速致盲的眼内感染之一：眼内炎。在这项研究中，芽孢杆菌属。从眼部感染病例中分离，通过Riboprint分析进行分类学表征，并筛选推定毒力因子的存在。这些分离株杀死视网膜和角膜细胞的能力进行了检查，作为抗生素敏感性谱。绝大多数菌株属于B型。蜡状菌属微生物分类群，鉴定为B。蜡状芽孢杆菌（53%）或B.苏云金杆菌（26%）。在大多数B中鉴定出毒素。苏云金杆菌和B.蜡状菌分离物。大多数B。cereus和B.苏云金杆菌在6h内杀死角膜和视网膜细胞。所有分离株对大多数测试的抗生素敏感，其中喹诺酮类和万古霉素是最有效的。这些发现代表了B的首次报道。苏云金杆菌是一种重要的眼部病原菌，B具有潜在的眼部毒性。cereus和B.苏云金芽孢杆菌分离，并确定抗生素，其对芽孢杆菌的疗效上级临床使用。
Bacillus causes one of the most rapidly blinding intraocular infections: endophthalmitis. In this study, Bacillus spp. were isolated from ocular infection cases, taxonomically characterized by riboprint analysis, and screened for the presence of putative virulence factors. The ability of these isolates to kill retinal and corneal cells was examined, as were antibiotic susceptibility profiles. The majority of isolates belonged to the B. cereus taxonomic group of microorganisms and were identified as B. cereus (53%) or B. thuringiensis (26%). Toxins were identified in most B. thuringiensis and B. cereus isolates. Most B. cereus and B. thuringiensis killed corneal and retinal cells within 6 h. All isolates were susceptible to most antibiotics tested, with quinolones and vancomycin being the most potent. These findings represent the first report of B. thuringiensis as an important ocular pathogen, demonstrates the potential ocular toxicity of B. cereus and B. thuringiensis isolates, and identifies antibiotics whose efficacy against Bacillus were superior to those used clinically.