Trpc2 is expressed in two olfactory subsystems, the main and the vomeronasal system of larval Xenopus laevis
Trpc2 is expressed in two olfactory subsystems, the main and the vomeronasal system of larval Xenopus laevis
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DOI：
10.1242/jeb.103465
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发表时间：
2014-07-01
影响因子：
2.8
通讯作者：
Manzini, Ivan
中科院分区：
文献类型：
--
作者：
Sansone, Alfredo;Syed, Adnan S.;Manzini, Ivan
在两栖动物中首次观察到主嗅上皮和犁鼻上皮的完全分离。相比之下，硬骨鱼具有单一的嗅觉表面，其中主要和犁鼻嗅觉系统的遗传成分是混合的。瞬时受体电位通道TRPC2是犁鼻神经元的标记物，存在于单个鱼类的感觉表面，但已经局限于陆地两栖动物，红腿蝾螈（Plethodon shermani）的犁鼻上皮。本文研究了一种水生两栖动物TRPC2的定位，并克隆了非洲爪蟾TRPC2基因。我们发现它在MOE和犁鼻上皮中都有表达。这是首次在四足动物的MOE中广泛表达trpc2。trpc2在MOE中的表达模式与MOE特异性v2rs的表达模式几乎无法区分，这表明它们在相同的神经元亚群中共表达。
Complete segregation of the main olfactory epithelium (MOE) and the vomeronasal epithelium is first observed in amphibians. In contrast, teleost fishes possess a single olfactory surface, in which genetic components of the main and vomeronasal olfactory systems are intermingled. The transient receptor potential channel TRPC2, a marker of vomeronasal neurons, is present in the single fish sensory surface, but is already restricted to the vomeronasal epithelium in a terrestrial amphibian, the red-legged salamander (Plethodon shermani). Here we examined the localization of TRPC2 in an aquatic amphibian and cloned the Xenopus laevis trpc2 gene. We show that it is expressed in both the MOE and the vomeronasal epithelium. This is the first description of a broad trpc2 expression in the MOE of a tetrapod. The expression pattern of trpc2 in the MOE is virtually undistinguishable from that of MOE-specific v2rs, indicating that they are co-expressed in the same neuronal subpopulation.