SSEL, a selective enrichment broth for simultaneous growth ofSalmonella enterica,Staphylococcus aureus,Escherichia coliO157:H7, andListeria monocytogenes
SSEL, a selective enrichment broth for simultaneous growth ofSalmonella enterica,Staphylococcus aureus,Escherichia coliO157:H7, andListeria monocytogenes
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SSEL，一种选择性增菌肉汤，用于同时生长肠沙门氏菌、金黄色葡萄球菌、大肠杆菌 O157:H7 和单核细胞增生李斯特氏菌
DOI：
10.1111/jfs.12837
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发表时间：
2020
影响因子：
2.4
通讯作者：
Suo Yujuan
中科院分区：
文献类型：
--
作者：
Qu Yang;Bai Yalong;Liu Yanhong;Zhou Changyan;Zhou Xiujuan;Zhang Donglai;Shi Chunlei;Suo Yujuan
肠沙门氏菌、金黄色葡萄球菌、大肠杆菌O157：H7和单核细胞增生性李斯特菌可能会污染类似类型的食物，并导致食源性疾病。本研究的目的是研制一种选择性富集液，用于同时富集肠沙门氏菌、金黄色葡萄球菌、大肠杆菌O157：H7和单核细胞增多性李斯特菌(SSEL)。所研制的SSEL发酵液不仅能在37℃、10~100cfu/m L接种量下培养18 小时，使目标病原菌达到5log10cfu/m l，而且能成功地支持生长速率相近的目标病原菌的同时浓缩，并能有效地抑制大多数非靶标细菌的生长。用人工污染试验和多重聚合酶链式反应相结合的方法证实SSEL的浓缩效果。综上所述，SSEL已被证明是一种在单一检测平台上检测这四种病原体的有前景的多重选择性浓缩肉汤。
Salmonella enterica,Staphylococcus aureus,Escherichia coliO157: H7, andListeria monocytogenesmay contaminate similar types of food and cause foodborne disease. The objective of this study was to develop a selective enrichment broth for simultaneous enrichment ofSalmonella enterica,Staphylococcus aureus,Escherichia coliO157: H7, andListeria monocytogenes(SSEL) using nalidixic acid, acriflavine, lithium chloride, and sodium cholate as selective agents. Developed SSEL broth not only enriched the target pathogens to 5 log10 CFU/ml after 18 hr incubation at 37°C with 10–100 CFU/mL of inoculation concentration, but also could successfully support the simultaneous enrichment of target pathogens with similar growth rates and inhibit the growth of most nontarget bacteria effectively. The enrichment effect of SSEL was confirmed by artificial contamination test coupled with multiplex PCR. In summary, SSEL has been shown to be a promising multiplex selective enrichment broth for the detection of the four pathogens on a single‐assay platform.