Assessment of microPET performance in analyzing the rat brain under different types of anesthesia: comparison between quantitative data obtained with microPET and ex vivo autoradiography
Assessment of microPET performance in analyzing the rat brain under different types of anesthesia: comparison between quantitative data obtained with microPET and ex vivo autoradiography
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DOI：
10.1016/j.neuroimage.2003.08.020
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发表时间：
2003-12-01
期刊：
影响因子：
5.7
通讯作者：
Watanabe, Y
中科院分区：
文献类型：
--
作者：
Matsumura, A;Mizokawa, S;Watanabe, Y
MicroPET（正电子发射断层扫描）已被用于小动物实验。然而，扫描的量化和最佳条件尚未建立。本研究的目的是比较microPET与大鼠脑切片离体放射自显影的结果，基于2-[F-18]氟-2-脱氧-D-葡萄糖（FDG）方法，并建立扫描的最佳条件。作为一个例子，我们检查了葡萄糖代谢的大鼠大脑下6种麻醉和清醒状态。大鼠脑的扫描条件是（1）使用4 mm厚的铅套，（2）350-650 keV的能量窗，和（3）6 ns的符合时间窗。在这些条件下，microPET的定量ROI数据与动物研究中FDG放射自显影的相应ROI数据显示出良好的相关性（r（2）= 0. 81）。在我们的方案中，当麻醉开始后40分钟注射FDG时，葡萄糖代谢几乎与清醒大鼠大脑中的葡萄糖代谢相同。(C)2003年爱思唯尔公司All rights reserved.
MicroPET (positron emission tomography) has been implemented for use in experiments with small animals. However, the quantification and optimal conditions for scanning are not established yet. The aim of this study was to compare the results obtained by microPET with those by ex vivo autoradiography of rat brain slices, based on the 2-[F-18]fluoro-2-deoxy-D-glucose (FDG) method, and to establish the optimal conditions for scanning. As an example, we examined glucose metabolism in the rat brain under 6 types of anesthesia and in the conscious state. The scanning conditions for the rat brain were (1) use of a 4-mm-thick leaden jacket, (2) an energy window of 350-650 keV, and (3) a coincidence time window of 6 ns. Under these conditions, the quantitative ROI data from microPET showed a good correlation with the corresponding ROI data from FDG autoradiography in the animal study (r(2) = 0. 81). With our protocol, when anesthesia was started 40 min after the FDG injection, the glucose metabolism was almost the same as that in the conscious rat brain. (C) 2003 Elsevier Inc. All rights reserved.