Molecular cloning of geranyl diphosphate synthase and compartmentation of monoterpene synthesis in plant cells
Molecular cloning of geranyl diphosphate synthase and compartmentation of monoterpene synthesis in plant cells
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DOI：
10.1046/j.1365-313x.2000.00875.x
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发表时间：
2000-10-01
期刊：
影响因子：
7.2
通讯作者：
Camara, B
中科院分区：
文献类型：
--
作者：
Bouvier, F;Suire, C;Camara, B
在植物中合成的类异戊二烯的性质主要由异戊烯基转移酶的特异性决定。这些酶中的几种已经在分子水平上表征。香叶基二磷酸（GPP）是参与植物防御、化感作用和授粉的无数单萜成分的碳骨架，其区室化和分子调控知之甚少。我们在这里描述的GPP合成酶（GPPS）从拟南芥的克隆和功能表达。不同的非分泌和分泌植物组织的免疫组织学分析表明，GPPS及其同系物，单萜合酶，脱氧木酮糖磷酸合酶和geranylgeranyldiphosphate合酶，是平等的区室化和分布在非绿色质体，以及在光合细胞的叶绿体。这表明单萜的合成不仅限于特殊的分泌结构，也可以发生在光合薄壁组织中。这些数据提供了新的信息，单萜生物合成是如何划分和诱导从头在不同的植物生物和非生物胁迫。
The nature of isoprenoids synthesized in plants is primarily determined by the specificity of prenyltransferases. Several of these enzymes have been characterized at the molecular level. The compartmentation and molecular regulation of geranyl diphosphate (GPP), the carbon skeleton that is the backbone of myriad monoterpene constituents involved in plant defence, allelopathic interactions and pollination, is poorly understood. We describe here the cloning and functional expression of a GPP synthase (GPPS) from Arabidopsis thaliana. Immunohistological analyses of diverse non-secretory and secretory plant tissues reveal that GPPS and its congeners, monoterpene synthase, deoxy-xylulose phosphate synthase and geranylgeranyl diphosphate synthase, are equally compartmentalized and distributed in non-green plastids as well in chloroplasts of photosynthetic cells. This argues that monoterpene synthesis is not solely restricted to specialized secretory structures but can also occur in photosynthetic parenchyma. These data provide new information as to how monoterpene biosynthesis is compartmentalized and induced de novo in response to biotic and abiotic stress in diverse plants.