Establishment of a Convenient System for the Culture and Study of Perineurium Barrier In Vitro
Establishment of a Convenient System for the Culture and Study of Perineurium Barrier In Vitro
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DOI：
10.1007/s10571-020-00978-0
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发表时间：
2020-10
影响因子：
4
通讯作者：
Xuemin Yin;Xiaohao Liu;Yan Zhang;Jiao Zeng;Xiaodan Liang;Xiaojun Yang;J. Hou
中科院分区：
文献类型：
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作者：
Xuemin Yin;Xiaohao Liu;Yan Zhang;Jiao Zeng;Xiaodan Liang;Xiaojun Yang;J. Hou
神经束膜作为周围神经系统中的选择性、代谢活跃的扩散屏障，其由通过紧密连接（TJ）连接在一起的神经束膜细胞组成。已知这些连接不仅在维持细胞极性和组织完整性方面发挥重要作用，而且还限制某些分子和离子的细胞旁扩散，而TJ屏障功能的丧失对于肿瘤生长、侵袭和转移是必要的。因此，对神经束膜细胞屏障功能的深入研究将有助于深入了解神经束膜侵袭（PNI）的分子机制。在这项研究中，我们的目的是开发一种有效的程序，建立神经束膜细胞系作为一种工具，调查周围神经屏障的生理和病理生理。在此，提出了在有利条件下神经束膜细胞系的分离、扩增、表征和维持。此外，这些神经束膜细胞的表型特征的分析以及PNI的研究屏障功能进行了描述。这些技术可以为神经束膜细胞的功能和分子研究提供有价值的手段，特别是可以阐明PNI和其他周围神经疾病的发病机制和进展。
The perineurium serves as a selective, metabolically active diffusion barrier in the peripheral nervous system, which is composed of perineurial cells joined together by tight junctions (TJs). Not only are these junctions known to play an essential role in maintaining cellular polarity and tissue integrity, but also limit the paracellular diffusion of certain molecules and ions, whereas loss of TJs barrier function is imperative for tumour growth, invasion and metastasis. Hence, a detailed study on the barrier function of perineurial cells may provide insights into the molecular mechanism of perineural invasion (PNI). In this study, we aimed to develop an efficient procedure for the establishment of perineurial cell lines as a tool for investigating the physiology and pathophysiology of the peripheral nerve barriers. Herein, the isolation, expansion, characterization and maintenance of perineurial cell lines under favourable conditions are presented. Furthermore, the analysis of the phenotypic features of these perineurial cells as well as the barrier function for the study of PNI are described. Such techniques may provide a valuable means for the functional and molecular investigation of perineurial cells, and in particular may elucidate the pathogenesis and progression of PNI, and other peripheral nerve disorders.