Mutagenic effects of carbon-ion irradiation on dry Arabidopsis thaliana seeds
Mutagenic effects of carbon-ion irradiation on dry Arabidopsis thaliana seeds
复制标题
碳离子辐照对拟南芥干种子的诱变效应
DOI：
10.1016/j.mrgentox.2013.07.018
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发表时间：
2014-01-01
影响因子：
1.9
通讯作者：
Zhou, Libin
中科院分区：
文献类型：
--
作者：
Du, Yan;Li, Wenjian;Zhou, Libin
为了研究碳离子对拟南芥（Columbia生态型）的诱变效应，分离植物发育中的有用基因，将43 MeV/u的碳离子分别暴露于0、100、200、300、400、500和600 Gy的碳离子环境中。对M-1植株的成活率、主根长度和下胚轴长度进行分析，选择200 Gy作为大规模试验的剂量。来自28,062个M-2群体的1363个品系表现出叶、茎、花或生命周期的改变，以叶片异常和生命周期提前为主要表型变异。5个稳定的可遗传突变的突变基因位点被粗略定位到染色体上。获得了新的突变体，尽管一些突变体与以往研究中乙基甲烷磺酸盐（EMS）诱导的突变体相似。本研究提供了大量描述碳离子辐照诱导拟南芥突变表型的具体信息。这些结果表明，碳离子束与其他诱变剂一样，在植物突变体育种中是有用和有效的，并且它们将使突变体育种更加多样化。(C) 2013 Elsevier B.V.版权所有
To investigate the mutagenic effects of carbon ions on Arabidopsis thaliana (ecotype Columbia) and to isolate useful genes in plant development, dry seeds were exposed to 43 MeV/u carbon ions at doses of 0, 100, 200, 300, 400, 500 and 600 Gy. The survival rate, primary root length, and hypocotyl length of M-1 plants were analyzed, and 200 Gy was selected as the dose for the large-scale experiment. A total of 1363 lines of plants from 28,062 M-2 populations displayed alterations in the leaf, stem, flower, or life cycle, with abnormal leaves and a premature life cycle as the main phenotypic variations. The mutated gene loci of five stable and inheritable mutations were roughly mapped on chromosomes. Novel mutants were obtained, although some of the mutants were similar to mutants induced by ethylmethane sulfonate (EMS) according to previous studies. This study provides a large body of specific information describing A. thaliana mutation phenotypes that were induced by carbon-ion irradiation. These results suggest that carbon-ion beams are as useful and effective as other mutagens for mutant breeding in plants, and that they will allow mutant breeding that is more diversified. (C) 2013 Elsevier B.V. All rights reserved.