Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea
Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea
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DOI：
10.2307/3761991
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发表时间：
2004-01-01
期刊：
影响因子：
2.8
通讯作者：
Wingfield, MJ
中科院分区：
文献类型：
--
作者：
Slippers, B;Crous, PW;Wingfield, MJ
Botryosphaeria dothidea 是木本植物重要病原体属中最常报道的物种之一。该分类单元通常被认为代表一个物种复合体，因此其身份仍不清楚。先前的研究要么将 B. dothidea 视为 B. ribis 和 B. berengeriana 的有效名称，要么认为它们是独立的实体。更令人困惑的是，没有可用于 B. dothidea 或 B. ribis 的前型培养物。因此，本研究的目的是重新收集和表征这些真菌，并指定一组可用于未来研究的参考培养物。为此，使用来自 rDNA（ITS 1、5.8S 和 ITS 2）、β-微管蛋白和 EF1-α 基因的形态学、文化和多等位基因 DNA 序列数据集来全面表征这些物种。人们发现 Botryosphaeria dothidea 与 B. ribis 不同，而 B. berengeriana 则保留为前一个名称的同义词。此外，Fusicaccum aesculi 被认为是 B. dothidea 的无性型，而 B. ribis 的无性型最近被描述为 F ribis sp。十一月根据β-微管蛋白和EF1-α序列数据，可以将Botryosphaeria ibis 与B. parva 区分开来。本研究中使用的三个基因区域的组合系统发育还表明，Botryosphaeria 属代表了两种不同的系统发育组合，分别对应于具有二倍体和无性无性梭菌的物种。
Botryosphaeria dothidea is one of the most commonly reported species in a genus of important pathogens of woody plants. This taxon generally is accepted to represent a species complex, and hence its identity remains unclear. Previous studies either have treated B. dothidea as the valid name for B. ribis and B. berengeriana or argued for them to be separate entities. To add to the confusion, no ex-type cultures are available for either B. dothidea or B. ribis. The aim of the present study, therefore, was to recollect and characterize these fungi and designate a set of reference cultures that can be used in future studies. To this end, morphological, cultural and multi-allelic DNA sequence datasets from the rDNA (ITS 1, 5.8S, and ITS 2), beta-tubulin and EF1-alpha genes were used to fully characterize these species. Botryosphaeria dothidea was found to be distinct from B. ribis, while B. berengeriana was retained as synonym of the former name. Furthermore, Fusicoccum aesculi is accepted as anamorph of B. dothidea, while the anamorph of B. ribis is newly described as F ribis sp. nov. Botryosphaeria ?ibis could be distinguished from B. parva based on beta-tubulin and EF1-alpha sequence data. A combined phylogeny of the three gene regions used in this study also showed that the genus Botryosphaeria represents two distinct phylogenetic assemblages that correspond to species with Diplodia and Fusicoccum anamorphs.