Anti-apoptotic effects of hyperoside via inhibition of NR2B-containing NMDA receptors
Anti-apoptotic effects of hyperoside via inhibition of NR2B-containing NMDA receptors
复制标题
金丝桃苷通过抑制含有 NR2B 的 NMDA 受体发挥抗凋亡作用
DOI：
10.1016/s1734-1140(10)70356-x
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发表时间：
2010-09-01
影响因子：
4.4
通讯作者：
Zhao, Ming-Gao
中科院分区：
文献类型：
--
作者：
Zhang, Xiao-Nan;Li, Jin-Mao;Zhao, Ming-Gao
金丝桃苷（Hyp）是一种从民间药物——欧洲杜鹃叶中分离出的黄酮类化合物。它已被证实在体内和体外均具有神经保护作用。然而，关于Hyp对谷氨酸诱导的神经元凋亡的影响知之甚少。本研究表明，Hyp以浓度依赖的方式显著减轻了培养的神经元暴露于N -甲基 - D -天冬氨酸（NMDA）所诱导的凋亡。蛋白质印迹分析显示，Hyp拮抗了含过量NR2B的NMDA受体的表达；然而，它对含NR2A的NMDA受体的表达没有影响。我们的结果表明，Hyp的神经保护作用至少部分归因于其对含NR2A和NR2B的NMDA受体的差异性调节。
Hyperoside (Hyp) is a flavonoid compound isolated from a folk remedy, Rhododendron ponticum L. leaves. It has been shown to have neuroprotective effects both in vivo and in vitro. However, little is known about the effects of Hyp on the neuronal apoptosis induced by glutamate. The present study showed that Hyp significantly attenuated, in a concentration-dependent manner, the apoptosis induced by the exposure of cultured neurons to NMDA. Western blot analysis revealed that Hyp antagonized the expression of excess NR2B-containing NMDA receptors; however, it had no effect on the expression of NR2A-containing NMDA receptors. Our results demonstrate that the neuroprotective effect of Hyp owes, at least partially, to its differential modulation of NR2A- and NR2B-containing NMDA receptors.