Yeast Ku as a regulator of chromosomal DNA end structure
Yeast Ku as a regulator of chromosomal DNA end structure
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DOI：
10.1126/science.280.5364.741
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发表时间：
1998-05-01
期刊：
影响因子：
56.9
通讯作者：
Wellinger, RJ
中科院分区：
文献类型：
--
作者：
Gravel, S;Larrivée, M;Wellinger, RJ
During telomere replication in yeast, chromosome ends acquire an S-phase-specific overhang of the guanosine-rich strand. Here it is shown that in cells lacking Ku, a heterodimeric protein involved in nonhomologous DNA end joining, these overhangs are present throughout the cell cycle. In vivo cross-linking experiments demonstrated that Ku is bound to telomeric DNA, These results show that Ku plays a direct role in establishing a normal DNA end structure on yeast chromosomes, conceivably by functioning as a terminus-binding factor. Because Ku-mediated DNA end joining involving telomeres would result in chromosome instability, our data also suggest that Ku;has a distinct function when bound to telomeres.
