Hybrid Polymer-Lipid Films as Platforms for Directed Membrane Protein Insertion
Hybrid Polymer-Lipid Films as Platforms for Directed Membrane Protein Insertion
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DOI：
10.1021/acs.langmuir.5b00388
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发表时间：
2015-05-05
期刊：
影响因子：
3.9
通讯作者：
Meier, Wolfgang
中科院分区：
文献类型：
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作者：
Kowal, Justyna;Wu, Dalin;Meier, Wolfgang
由两亲嵌段共聚物和脂类组成的杂化材料构成了新一代生物膜激励材料。由脂类和聚合物自组装而成的杂化膜代表了人工膜和天然膜之间相互作用的可调节模型，这对于开发药物输送系统具有关键意义。通过将poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline)-b-PMOXA两亲性共聚物与不同的磷脂结合，我们得到了基于相分离和形成不同结构域的具有调制性质和拓扑结构的杂化薄膜。通过了解驱动这些脂质-聚合物杂化膜相分离的因素，我们能够将它们用作膜蛋白定向插入的平台。调节聚合物-脂类混合物的组成有利于成功插入具有所需拓扑分布的膜蛋白(在聚合物或/和脂类区域)。膜蛋白在杂化薄膜中的受控插入和定位使这些杂化材料成为许多需要特定空间功能的应用的理想候选者。
Hybrids composed of amphiphilic block copolymers and lipids constitute a new generation of biological membrane-inspired materials. Hybrid membranes resulting from self-assembly of lipids and polymers represent adjustable models for interactions between artificial and natural membranes, which are of key importance, e.g., when developing systems for drug delivery. By combining poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) amphiphilic copolymers (PDMS-b-PMOXA) with various phospholipids, we obtained hybrid films with modulated properties and topology, based on phase separation, and the formation of distinct domains. By understanding the factors driving the phase separation in these hybrid lipid-polymer films, we were able to use them as platforms for directed insertion of membrane proteins. Tuning the composition of the polymer-lipids mixtures favored successful insertion of membrane proteins with desired topological distributions (in polymer or/and lipid regions). Controlled insertion and location of membrane proteins in hybrid films make these hybrids ideal candidates for numerous applications where specific spatial functionality is required.