Study of γ-H2AX as DNA double strand break biomarker in resident living in high natural radiation area of Mamuju, West Sulawesi
Study of γ-H2AX as DNA double strand break biomarker in resident living in high natural radiation area of Mamuju, West Sulawesi
复制标题
DOI：
10.1016/j.jenvrad.2017.02.012
复制
发表时间：
2017-05-01
影响因子：
2.3
通讯作者：
Syaifudin, Mukh
中科院分区：
文献类型：
--
作者：
Basri, Iin Kurnia Hasan;Yusuf, Darlina;Syaifudin, Mukh
磷酸组蛋白γ-H2 AX（双链DNA损伤的敏感标志物）的高表达被认为是可能导致突变并最终导致癌症的缺陷DNA修复途径或基因组不稳定性的指示。DNA损伤可能由电离辐射暴露引起。除了在医疗/诊断或工业中，电离辐射照射也可以在天然本底辐射高的地区自然发现。在这项研究中，我们收集了45名志愿者的血液，生活在马木居，在印度尼西亚的天然辐射最高的地区（剂量-7 mSv/年）。受试者分为高自然背景区（HNBA）（n = 37）和对照区（n = 8）。通过一种研究者荧光显微镜检查来评估γ-H2 AX表达灶。我们的结果表明，每个细胞的平均病灶数在正常范围内。虽然没有统计学差异，但暴露区域中的γ-H2 AX病灶的平均值在暴露区域中高于对照区域，分别为0.31对0.13（p > 0.05）。接触区与对照区男女、老年人与青年人γ-H_2AX平均病灶数差异无统计学意义（p > 0.05）。在这项初步研究中，我们发现，γ-H2 AX病灶（从而DNA双链断裂）频率在居民生活在HNBA的马木居，西苏拉威西，显示出较高的趋势（虽然不显着）相对于控制区的平均值。需要更多的研究来进一步审查这些观察结果。(C)2017爱思唯尔有限公司版权所有
High expression of phospho histone gamma-H2AX, a sensitive marker of double stranded DNA damage, is believed to be an indication of defective DNA repair pathway or genomic instability that may cause mutations and ultimately cancer. DNA damage can be caused by ionizing radiation exposure. Beside in medical treatment/diagnosis or industry, ionizing radiation exposure can also be found in naturally in regions of high natural back ground radiation. In this study we collect the blood from 45 volunteers living in Mamuju, a region with highest natural radiation in Indonesia (dose of -7 mSv/year). Subjects were grouped as high natural background area (HNBA) (n = 37) and control area (n = 8). The expression gamma-H2AX foci were evaluated by one of researcher fluorescence microscope examination. Our results show that the average foci numbers per cell were in the normal range. While not statistical different, the average of gamma-H2AX foci in exposed area higher in the exposed compared to the control area, 031 versus 0.13 (p > 0.05), respectively. Moreover, there was also no statistical difference of average gamma-H2AX foci between man and woman, old and young people in exposed and control area (p > 0.05). In this pre-liminary study we find that gamma-H2AX foci (and thus DNA double strand break) frequency in residents living in the HNBA of Mamuju, West Sulawesi, show a trend towards higher (albeit not significant) average values relative to the control area. More research is needed to further scrutinize these observations. (C) 2017 Elsevier Ltd. All rights reserved.