A biomimetic approach for the creation of two-dimensional microscale surface patterns: creation of isolated immunological synapses.
A biomimetic approach for the creation of two-dimensional microscale surface patterns: creation of isolated immunological synapses.
复制标题
创建二维微尺度表面图案的仿生方法：创建孤立的免疫突触。
DOI：
10.1155/2009/821308
复制
发表时间：
2009
影响因子：
3.1
通讯作者：
Fahmy,TarekM
中科院分区：
文献类型：
--
作者：
Stern,Eric;Mooney,DavidJ;Fahmy,TarekM
目前在表面功能化方面的努力还没有产生能够产生由多种蛋白质组成的特定二维微尺度几何阵列的稳健技术。这种能力对于在传感和细胞图案化应用中工程化基底是期望的，其中需要特定构型的至少两种不同蛋白质功能。在这里，我们介绍了一种新的方法来创建阵列的微尺度几何形状。我们展示了我们的方法与仿生结构的灵感来自免疫突触，细胞-细胞界面结构的特点是两个同心环的蛋白质：外部粘附蛋白质结构和内部识别配体核心。该技术的强大之处在于它能够以任何定义的几何形状对任何蛋白质进行图案化，以及并行创建阵列。
Current efforts in surface functionalization have not produced a robust technique capable of creating specific two‐dimensional microscale geometrical arrays composed of multiple proteins. Such a capability is desirable for engineering substrates in sensing and cell patterning applications where at least two different protein functionalities in a specific configuration are required. Here we introduce a new approach for the creation of arrays of microscale geometries. We demonstrate our approach with a biomimetic structure inspired by the immunological synapse, a cell‐cell interfacial structure characterized by two concentric rings of proteins: an outer adhesion protein structure and an inner recognition ligand core. The power of the technique lies in its ability to pattern any protein in any defined geometry as well as to create arrays in parallel.