The effect of metabolic inhibitors on transferrin and iron uptake and transferrin release from reticulocytes.
The effect of metabolic inhibitors on transferrin and iron uptake and transferrin release from reticulocytes.
复制标题
代谢抑制剂对转铁蛋白和网织红细胞铁摄取和转铁蛋白释放的影响。
DOI：
10.1016/0304-4165(69)90048-8
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发表时间：
1969
期刊：
影响因子：
--
通讯作者：
E. Baker
中科院分区：
文献类型：
--
作者：
E. Morgan;E. Baker
根据代谢抑制剂对兔网织红细胞摄取[~(125)I]转铁蛋白和~(59)Fe和释放[~(125)I]转铁蛋白的影响大致分为三类：1.1。对转铁蛋白或铁摄取没有影响的抑制剂(丙二酸钠和哇巴因)。阻止铁摄取大于转铁蛋白摄取的药物(维酮、2，4-二硝基苯酚、寡霉素、NaCN、NaN3、NaF和放线菌亚胺)。这些化合物的作用归因于对线粒体电子转移链的抑制。对铁和转铁蛋白摄取的抑制程度大致相同(亚砷酸钠、碘乙酰胺、N-乙基马来酰亚胺、对氯汞苯甲酸盐(PCMB)、对氯汞苯磺酸(PCMBS)和醋酸铅)。所研究的所有抑制剂均立即起抑制作用，但预先加入摩尔浓度为抑制剂摩尔浓度1-4倍的半胱氨酸，可在很大程度上阻止碘乙酰胺、N-乙基马来酰亚胺、PCMB和PCMBS的作用。亚砷酸钠、碘乙酰胺和N-乙基马来酰亚胺也抑制转铁蛋白的释放。这些化合物(所有的巯基试剂)可能通过改变网织红细胞的膜结构，减少转铁蛋白，从而减少铁的摄取而起作用。
Metabolic inhibitors were broadly classified into three groups on the basis of their effect on the uptake of [125I]transferrin and59Fe and the release of [125I]transferrin by rabbit reticulocytesin vitro:1.1. Inhibitors which had no effect on transferrin or iron uptake (sodium malonate and ouabain).2.2. Those which blocked iron uptake more than transferrin uptake (retonone, 2,4-dinitrophenol, oligomycin, NaCN, NaN3, NaF and cycloheximide). The effect of these compounds was attributed to inhibition of the mitochondrial electron transfer chain.3.3. Those which inhibited iron and transferrin uptake to approximately the same degree (sodium arsenite, iodoacetamide,N-ethylmaleimide,p-chloromercuribenzoate (PCMB),p-chloromercuribenzenesulfonate (PCMBS) and lead acetate). The onset of inhibition by all the inhibitors studied was immediate, but the effects of iodoacetamide,N-ethylmaleimide, PCMB and PCMBS were largely prevented by prior addition of cysteine in 1–4 times the molar concentration of inhibitor. Sodium arsenite, iodoacetamide andN-ethylmaleimide also inhbited transferrin release. These compounds (all sulfhydryl reagents) may act by altering reticulocyte membrane structure, reducing transferrin and hence iron uptake.