Klhl31 is associated with skeletal myogenesis and its expression is regulated by myogenic signals and Myf-5
Klhl31 is associated with skeletal myogenesis and its expression is regulated by myogenic signals and Myf-5
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DOI：
10.1016/j.mod.2009.07.006
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发表时间：
2009-10-01
影响因子：
2.6
通讯作者：
Muensterberg, Andrea
中科院分区：
文献类型：
--
作者：
Abou-Elhamd, Alaa;Cooper, Oliver;Muensterberg, Andrea
Klhl31是Kelch果蝇的同源物，属于脊椎动物Kelch样蛋白家族。该家族的成员含有多个蛋白质结构域，包括氨基末端宽复合体/电轨道/砖-一-支架（BTB）或痘病毒和锌指（POZ）结构域、羧基末端Kelch重复序列和中心连接区域。我们发现Klhl31在发育中的心脏、小体肌组以及后来分化的骨骼肌和心肌中高度表达。在发育过程中，Klhl31在骨骼肌特异性bHLH转录因子MyoD首次表达后不久，在肌组的外轴结构域开始表达。Klhl31在整个胚胎和胎儿发育过程中都在骨骼肌中表达。组织消融和恢复实验调节肌肉发生也控制体细胞中Klhl31的表达。特别是轴向组织、神经管、底板和脊索以及表面外胚层，为肌肉发生和Klhl31的适当表达提供了组合线索。我们发现，肌源性信号Shh和Wnt-1或Wnt-6的组合足以在背体中表达Klhl31。此外，Myf-5的异位表达导致Klhl31在发育中的神经管中表达，这表明Klhl31是脊椎动物肌发生的一个新的组成部分。2009爱思唯尔爱尔兰有限公司版权所有。
Klhl31 is an orthologue of Drosophila Kelch and belongs to a family of Kelch-like proteins in vertebrates. Members of this family contain multiple protein domains, including an aminoterminal broad complex/tram-track/bric-a-brac (BTB) or poxvirus and zinc finger (POZ) domain, carboxy-terminal Kelch repeats and a central linker region. We show that Klhl31 is highly expressed in the developing heart, the somite myotome and later in differentiated skeletal muscle and the myocardium. In developing somites expression of Klhl31 was initiated in the epaxial domain of the myotome, shortly after the skeletal muscle specific bHLH transcription factor, MyoD, was first expressed. Klhl31 remained expressed in skeletal muscle throughout embryonic and fetal development. Tissue ablations and rescue experiments that regulate myogenesis also govern expression of Klhl31 expression in somites. In particular, axial tissues, neural tube, floor plate and notochord, and surface ectoderm, provide combinatorial cues for myogenesis and the appropriate expression of Klhl31. We show that a combination of myogenic signals, Shh and either Wnt-1 or Wnt-6, are sufficient for Klhl31 expression in the dorsal somite. Furthermore, ectopic expression of Myf-5 led to expression of Klhl31 in the developing neural tube, indicating that Klhl31 is a novel and integral part of vertebrate myogenesis. (C) 2009 Elsevier Ireland Ltd. All rights reserved.