Gut immune stimulation by non pathogenic Gram(+) and Gram(-) bacteria.: Comparison with a probiotic strain
Gut immune stimulation by non pathogenic Gram(+) and Gram(-) bacteria.: Comparison with a probiotic strain
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DOI：
10.1016/j.cyto.2007.11.014
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发表时间：
2008-03-01
期刊：
影响因子：
3.8
通讯作者：
Perdigon, Gabriela
中科院分区：
文献类型：
--
作者：
Dogi, C. A.;Galdeano, C. Maldonado;Perdigon, Gabriela
我们分析了革兰氏阳性菌诱导的肠道免疫刺激：非益生菌嗜酸乳杆菌CRL 1462和嗜酸乳杆菌A9；两种潜在的益生菌菌株：嗜酸乳杆菌CRL 924和德尔布鲁氏乳杆菌亚种。bulgaricus CRL 423；与一种益生菌菌株：干酪乳杆菌CRL 431比较。我们还研究了BALB/c小鼠的革兰氏阴性菌：大肠杆菌129和大肠杆菌13-7。所有菌株的IgA+细胞数量均增加。我们分析了细胞因子IFN γ， tnf - α, IL-17。IL-12， IL-6和MIP-1。Gram（+）菌株增加了IL-10+细胞的数量。Gram（-）菌株没有增加IL-10+细胞，但增加了IL-12+细胞的数量。益生菌菌株主要增加IFN γ和TNF α。在对受体TLR-2、TLR-4和CD-206的研究中，我们发现只有益生菌菌株增加了CD-206+细胞的数量。所有Gram（+）菌株均增加了TLR-2+细胞的数量，而Gram（-）菌株增加了TLR-4+细胞的数量。益生菌菌株通过富集于肠上皮细胞（IEC）的制剂诱导IL-6的释放。革兰氏（+）和革兰氏（-）细菌激活不同的免疫受体并诱导不同的细胞因子谱。该菌株对免疫细胞和IEC富集菌群具有较强的活性，主要激活先天免疫系统细胞。(c) 2007 Elsevier Ltd.版权所有。
We analyzed the gut immune stimulation induced by Gram-positive bacteria: non probiotic Lactobacillus acidophilus CRL 1462 and Lactobacillus acidophilus A9; two potentially probiotic strains: L. acidophilus CRL 924 and Lactobacillus delbrueckii subsp. bulgaricus CRL 423; comparatively with a probiotic strain: Lactobacillus casei CRL 431. We also studied Gram-negative bacteria: Escherichia coli 129 and E coli 13-7 in BALB/c mice. All the strains increased the number of IgA+ cells. We analyzed the cytokines IFN gamma, TNF-alpha, IL-17. IL-12, IL-6 and MIP-1 alpha. The Gram(+) strains increased the number of IL-10+ cells. Gram(-) strains did not increase IL-10+ cells, but they increased the number of IL-12+ cells. The probiotic strain increased mainly IFN gamma and TNF alpha. In the study of the receptors TLR-2, TLR-4 and CD-206, we demonstrated that only the probiotic strain increased the number of CD-206+ cells. All the Gram(+) strains increased the number of TLR-2+ cells and the Gram(-) strains of the TLR-4+ cells. The probiotic strain induced the release of IL-6 by a preparation enriched in intestinal epithelial cells (IEC). Gram(+) and Gram(-)) bacteria activated different immune receptors and induced a different cytokine profile. The probiotic strain showed a great activity on the immune cells and the enriched population in IEC, activating mainly cells of the innate immune system. (c) 2007 Elsevier Ltd. All rights reserved.