Effects of dietary broccoli and butylated hydroxyanisole on liver-mediated metabolism of benzo[a]pyrene.
Effects of dietary broccoli and butylated hydroxyanisole on liver-mediated metabolism of benzo[a]pyrene.
复制标题
膳食西兰花和丁基羟基苯甲醚对肝脏介导的苯并[a]芘代谢的影响。
DOI：
10.1016/0278-6915(83)90227-2
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
L. Bjeldanes
中科院分区：
文献类型：
--
作者：
K. Aspry;L. Bjeldanes
雄性Wistar大鼠组给予基础饮食或添加10或25%花椰菜或0.8% BHA的饮食。肝组分进行了细胞色素P-450，芳烃羟化酶（AHH），谷胱甘肽-S-转移酶和环氧化物水解酶的活性和苯并[a]芘（BaP）代谢。还进行了肝微粒体的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳。BHA组的平均相对肝脏重量显著高于对照组和10%西兰花组，但不显著高于25%西兰花组。肝微粒体的凝胶电泳表明，在对应于某些cytochromeP-450的分子量的条带中，强度存在饮食依赖性变化。在给予西兰花补充饮食的大鼠肝脏中观察到细胞色素P-450和AHH、谷胱甘肽-S-转移酶和环氧化物水解酶活性的饮食依赖性增加（比对照组增加20-90%）。除AHH活性外，这种增加也发生在BHA组。BaP代谢物的分析显示，相对于对照组，西兰花或BHA处理组中形成的4，5-二醇相对于鉴定的主要二醇的比例没有变化。青花菜喂养组中形成的9，10-二醇的比例没有变化，但BHA组中形成的9，10-二醇的比例显著高于对照组。在两个西兰花喂养组中形成的顺式和反式-7，8-二醇的比例没有变化，但在BHA组中显着降低。与对照组相比，苯酚I（主要包括9-OH-BaP）与总苯酚（主要是9-OH和3-OH）的比率在25%-花椰菜组中显著降低约30%，在BHA组中显著降低约70%。还观察到来自对照组和25%西兰花和BHA喂养大鼠的样品之间苯酚-II峰（包括3-OH和7-OH苯酚）的定性差异。这些研究结果的影响进行了讨论西兰花和BHA苯并[a]芘毒性的影响。
Groups of male Wistar rats were given either a basal diet or diets supplemented with 10 or 25% broccoli or 0.8% BHA. Liver fractions were assayed for cytochromeP-450, for aryl hydrocarbon hydroxylase (AHH), glutathione-S-transferase and epoxide hydrolase activities and for benzo[a]pyrene (BaP) metabolism. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of liver microsomes was also performed. Mean relative liver weight in the BHA group was significantly higher than that of the control and 10% broccoli groups but not significantly higher than that of the 25% broccoli group. Gel electrophoresis of liver microsomes indicated a diet-dependent variation in intensity in a band that corresponded in mol wt to those of certain cytochromeP-450s. Diet-dependent increases (20–90% over control) in cytochromeP-450 and in the activities of AHH, glutathione-S-transferase and epoxide hydrolase were observed in livers from rats given broccoli-supplemented diets. Except for AHH activity, such increases also occurred in the group fed BHA. Analysis of BaP metabolites revealed that the proportion of 4,5-diol formed relative to the major diols identified was unchanged in the broccoli- or BHA-treated groups relative to the control group. The proportion of 9,10-diol formed was unchanged in the broccoli-fed groups but was significantly higher in the BHA group than in the control group. The proportion ofcisandtrans-7,8-diol formed was unchanged in both broccoli-fed groups but was significantly lower in the BHA group. In comparison with the control group, the ratio of phenol I (comprising primarily 9-OH-BaP) to total phenols (primarily 9-OH and 3-OH) was significantly decreased by about 30% in the 25%-broccoli group and by about 70% in the BHA group. Qualitative differences in the phenol-II peak (comprising 3-OH and 7-OH phenols) were also observed between samples from the controls and those of 25%-broccoli- and BHA-fed rats. The implications of these findings are discussed with respect to the effects of broccoli and BHA on benzo[a]pyrene toxicity.