A measure of the mitotic index:: studies of the abundance and half-life of p34cdc2 in cultured cells and normal and neoplastic tissues
A measure of the mitotic index:: studies of the abundance and half-life of p34cdc2 in cultured cells and normal and neoplastic tissues
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DOI：
10.1046/j.1365-2443.1998.00163.x
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发表时间：
1998-01-01
期刊：
影响因子：
2.1
通讯作者：
Hunt, T
中科院分区：
文献类型：
--
作者：
Gannon, JV;Nebreda, A;Hunt, T
背景：cdc2基因编码一种蛋白激酶p34（cdc2），它是有丝分裂所必需的，并且在分裂细胞中以高水平存在。这种蛋白在分裂和静止细胞中的水平的经典研究使用与CDK家族的其他成员，特别是与CDK2交叉反应的抗体。因此，我们使用不与CDK2反应的高度特异性表位定位单克隆抗体重新检查了多种组织和细胞系中p34（cdc2）的丰度。我们在增殖细胞中观察到高水平的p34（cdc2），特别是在肿瘤组织中。退出细胞周期的细胞具有低水平或不可检测的水平。在有丝分裂结束时，p34（CDC2）的CHE水平以简单的一级动力学下降，半衰期从不小于6小时，更典型地为约18小时。结论：p34（cdc2）的免疫组化检测可准确、可靠、有意义地反映组织中细胞的增殖活性，p34（cdc2）可作为有丝分裂指数的最可靠的分子标记。
Background: The cdc2 gene encodes a protein kinase, p34(cdc2), that is essential for mitosis, and is present at high levels in dividing cells. Classical studies of the levels of this protein in dividing and resting cells used antibodies that cross-react with other members of the CDK family, in particular with CDK2. We have therefore re-examined the abundance of p34(cdc2) in a variety of tissues and cell lines, using a highly specific, epitope-mapped monoclonal antibody that does not react with CDK2.Results: We observed high levels of p34(cdc2) in proliferating cells, especially those in neoplastic tissues, Cells that have withdrawn from the cell cycle have low or undetectable levels, At the end of mitosis, che level of p34(cdc2) declines, with simple first-order kinetics, with a half-life which is never less than 6h and is more typically about 18h. The persistence of p34(cdc2) after the last cell division is comparable to that of PCNA, a commonly used marker of proliferation.Conclusions: The immunochemical detection of p34(cdc2) provides an accurate, reliable and meaningful measure of the proliferative activity of cells in tissues, We suggest that p34(cdc2) should be considered as the most authentic molecular marker of the mitotic index.