Crosslinking of chromosomal proteins to DNA in HeLa cells by UV gamma radiation and some antitumor drugs.
Crosslinking of chromosomal proteins to DNA in HeLa cells by UV gamma radiation and some antitumor drugs.
复制标题
通过紫外线伽马辐射和一些抗肿瘤药物，HeLa 细胞中染色体蛋白与 DNA 交联。
DOI：
10.1016/0006-291x(83)90847-1
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发表时间：
1983
影响因子：
3.1
通讯作者：
Stein,G
中科院分区：
文献类型：
--
作者：
Banjar,ZM;Hnilica,LS;Briggs,RC;Stein,J;Stein,G
用免疫化学技术研究了紫外线（UV）和γ射线以及1，3-二（2-氯乙基）-1-亚硝基脲（BCNU）或顺式和反式二氨二氯铂II（cis-DDP和trans-DDP）对蛋白质-DNA交联的影响。采用抗HeLa细胞核0.35 M NaCl提取物和0.35 M NaCl残留物的抗血清。γ和紫外线照射，暴露于顺式或反式DDP，并在较小程度上，BCNU，导致各种抗原的DNA交联。尽管几种抗原被所有使用的试剂交联，但其他抗原表现出试剂特异性交联模式。
Immunochemical techniques were used to investigate the protein-DNA crosslinking by ultraviolet (UV) and gamma radiation as well as 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) or cis- and trans-diamminedichloroplatinum II (cis-DDP and trans-DDP). Antisera to 0.35 M NaCl extract and 0.35 M NaCl residue of HeLa nuclei were employed. Both gamma and UV irradiation, exposure to cis- or trans-DDP and, to a lesser extent, BCNU, resulted in crosslinking of various antigens to the DNA. Although several antigens were crosslinked by all the employed agents, other exhibited agent-specific crosslinking patterns.