Experimental Cell Biology of Taste and Olfaction: Current Techniques and Protocols-猫眼课题宝

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发表时间：

1995-07

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作者：

A. Spielman;J. Brand

安乐死化学感官研究的一般方法，I.Ramirez鱼类和两栖动物味觉组织收集，J.H.Teeter哺乳动物味觉组织收集，A.I.Spielman和J.G.Brand昆虫：嗅觉感受器的分离和淋巴感受器的收集，G.Ziegelberger嗅觉器官的手术解剖和脊椎动物的组织收集方法，T.Kurahashi和D.Restrepo人类嗅觉组织的收集，L.D.Lowry和E.A.Pribitkin手术切除犁鼻组织及其验证，C.J.Wysocki和L.M.Wysocki ddnervation，B.Oakley舌片的制备，B.Oakley用于脊椎动物嗅觉感受器神经元的膜片钳电生理研究的细胞分离方法，大鼠味蕾组织培养的A.H.Kahn和S.Firestein，C.Ruiz，M.McPheters和S.C.Kinnamon嗅觉感受器神经元的G.V.Ronnett方法在嗅觉研究中空气和水刺激传递的方法，J.S.Kauer和S.Firestein在化学感官研究中的成像方法，I.J.Miller和S.L.HayesH.H.Crowley和J.C.Kinnamon嗅觉上皮扫描和透射电子显微镜，B.Ph.M.Menco通过连续切片和三维重建分析味蕾超微结构，S.M.Royer和J.C.Kinnamon免疫组织化学，C.DellaCorte IP3味觉感受器细胞放射自显影，P.A.Hwang原位杂交，H.Schmer和M.Behrens在化学感觉研究膜制备和亚细胞分裂中的生化方法，D.L.Kalinoski嗅觉纤毛的制备，R.R.H.Anholt作为化学感觉生化研究的工具，R.H.AnholtH.G.TRapido-Rosenthal受体结合和增溶，D.L.Kalinoski Western blotting，R.Bruch和Fe.用于监测第二信使信号的快速动力学的Abogadie方法，E.Tareilus，I.Boekhoff，A.I.Spielman和H.Breer Second Messenger Assay，A.I.Spielman，H.Nagai，G.Sunavala，M.Dasso，T.Huque，和J.G.Brand在化学感觉研究中的电生理和生物物理方法研究一般方法平面脂质双层中化学感受器蛋白的重建，A.M.Feigin，M.Zviman和J.H.Teeter在电生理学中的数据获取，V.E.Dionne全神经和单单位记录从电压钳制感受野中记录味觉神经，J.A.DeSimone，J.A.DeSimoneJ.Heck和Q.Ye蛙的全神经和单纤维记录，T.Miyamoto和T.Sato单单位电生理记录甲壳类化学感受器神经元的记录，C.D.德比在鱼类中的嗅觉和味觉记录，J.Caprio啮齿动物的味觉神经记录，M.E.Frank啮齿动物的三叉神经记录，B.P.Bryant在非人类灵长类动物中的全神经和单纤维记录，G.Hellekant和T.W.Roberts对犁鼻器和副嗅觉系统的电生理学记录，M.Meredith膜片钳夹泥犬味觉细胞的电生理学T.A.Cummings和S.C.Kinnamon斑片钳记录斑点叉尾轮鱼和虎蜥蜴幼体的味觉感受器细胞，J.H.Teeter对仓鼠和大鼠味觉细胞的斑片钳制，T.A.Gilbertson对小鼠味觉细胞的斑片钳制，J.F.MacDonald，I.Mody，M.W.Salter和A.I.Spielman现场松散斑片记录，B.Linden斑片钳制龙虾嗅觉细胞，B.W.AChe，D.A.Fadool和A.Zhainazarov全嗅觉Cilia的斑片钳制，B.W.Ach，D.A.Fadool，A.Zhainazarov用于脊椎动物嗅觉感受器细胞的Kleene膜片钳技术，G.Lowe，T.Nakamura和G.H.在昆虫嗅觉器官中的金单位和触角图记录，K.-E.Kaissling膜片钳昆虫中的嗅觉细胞，F.Zufall成像在化学感觉感受器细胞中的细胞内钙，D.Restrepo，M.Zviman和N.E.Rawson Visorate Imagorate Imagorate of电位法染料在嗅觉研究中的应用，J.S.Kauer方法研究嗅觉中的流动动力学，P.W.Scherer，K.Keyhani，I.Hahn，和M.Mozell分子生物学在化学感觉研究中的应用味觉cDNA的克隆及其表达模式的分析，P.J.McKinnon，S.K.McLaughlin和R.F.MarGolskee克隆气味受体，E.R.利曼和L.B.巴克未涵盖的技术的关键参考文献列表

General Methods in Chemosensory Research Euthanasia, I. Ramirez Collection of Taste Tissue from Fish and Amphibians, J. H. Teeter Collection of Taste Tissue from Mammals, A. I. Spielman and J. G. Brand Insects: Isolation of Olfactory Sensilla and Collection of Sensillum Lymph, G. Ziegelberger Surgical Anatomy of Olfactory Organ and Tissue Collection Methods in Vertebrates, T. Kurahashi and D. Restrepo Collection of Human Olfactory Tissue, L. D. Lowry and E. A. Pribitkin Surgical Removal of the Vomeronasal Organ and Its Verification, C. J. Wysocki and L. M. Wysocki Denervation and Reinnervation of the Tongue, B. Oakley The Lingual Slice Preparation, Isolated Taste Cells, and Dye Injections in Mudpuppy, A. Bigiani, D. A. Ewald, and S. D. Roper Cell Dissociation Methods for Patch Clamp Electrophysiological Studies of Vertebrate Olfactory Receptor Neurons, A. H. Kahn and S. Firestein Tissue Culture of Rat Taste Buds, C. Ruiz, M. McPheeters, and S. C. Kinnamon Olfactory Receptor Neuronal Cultures, G. V. Ronnett Methods for Airborne and Aqueous Stimulus Delivery in Olfactory Research, J. S. Kauer and S. Firestein Imaging Methods in Chemosensory Research Videomicroscopy and Image Processing, I. J. Miller and S. L. Hayes Transmission Electron Microscopy of Gustatory Epithelium, H. H. Crowley and J. C. Kinnamon Scanning and Transmission Electron Microscopy of Olfactory Epithelia, B. Ph. M. Menco Analysis of Taste Bud Ultrastructure by Serial Sectioning and Three-Dimensional Reconstruction, S. M. Royer and J. C. Kinnamon Immunohistochemistry, C. DellaCorte IP3 Autoradiography of Taste Receptor Cells, P. A. Hwang In Situ Hybridization, H. Schmale and M. Behrens Biochemical Methods in Chemosensory Research Membrane Preparation and Subcellular Fractionation, D. L. Kalinoski Preparation of Olfactory Cilia, R. R. H. Anholt The Use of the Spiny Lobster as a Tool for the Study of Chemosensory Biochemistry, H. G. Trapido-Rosenthal Receptor Binding and Solubilization, D. L. Kalinoski Western Blotting, R. Bruch and Fe. C. Abogadie Approaches for Monitoring Rapid Kinetics of Second Messenger Signaling, E. Tareilus, I. Boekhoff, A.I. Spielman, and H. Breer Second Messenger Assays, A. I. Spielman, H. Nagai, G. Sunavala, M. Dasso, T. Huque, and J. G. Brand Electrophysiological and Biophysical Methods in Chemosensory Research General Methods Reconstitution of Chemoreceptor Proteins in Planar Lipid Bilayers, A. M. Feigin, M. Zviman, and J. H. Teeter Data Acquisition in Electrophysiology, V. E. Dionne Whole Nerve and Single Unit Recordings Taste Nerve Recording from a Voltage-Clamped Receptive Field, J. A. DeSimone, G. Heck, and Q. Ye Whole Nerve and Single Fiber Recordings in Frog, T. Miyamoto and T. Sato Single Unit Electrophysiological Recordings from Crustacean Chemoreceptor Neurons, C. D. Derby Olfactory and Taste Recordings in Fish, J. Caprio Taste Nerve Recordings in Rodents, M. E. Frank Trigeminal Nerve Recordings in Rodents, B. P. Bryant Whole Nerve and Single Fiber Recordings in Non-Human Primates, G. Hellekant and T. W. Roberts Electrophysiology of the Vomeronasal Organ and Accessory Olfactory System, M. Meredith Patch-Clamping Patch-Clamping of Taste Cells in the Mudpuppy, T. A. Cummings and S. C. Kinnamon Patch-Clamping Recording from Taste Receptor Cells of Channel Catfish and Larval Tiger Salamander, J. H. Teeter Patch-Clamping of Taste Cells in Hamster and Rat, T. A. Gilbertson Patch-Clamping of Taste Cells in Mouse, J. F. MacDonald, I. Mody, M. W. Salter, and A. I. Spielman Loose-Patch Recording from Taste Buds in Situ, B. Lindemann Patch-Clamping Lobster Olfactory Receptor Cells, B. W. Ache, D. A. Fadool, and A. Zhainazarov Patch-Clamping of Whole Olfactory Cilia, S. J. Kleene Patch-Clamping Techniques for Vertebrate Olfactory Receptor Cells, G. Lowe, T. Nakamura, and G. H. Gold Single Unit and Electroantennogram Recordings in Insect Olfactory Organs, K.-E. Kaissling Patch-Clamping of Olfactory Cells in Insects, F. Zufall Imaging of Intracellular Calcium in Chemosensory Receptor Cells, D. Restrepo, M. Zviman, and N. E. Rawson Videorate Imaging of Potentiometric Dyes in Olfactory Research, J. S. Kauer Methods to Study Flow Dynamics in Olfaction, P. W. Scherer, K. Keyhani, I. Hahn, and M. Mozell Applications of Molecular Biology to Chemosensory Research Cloning of Gustatory cDNAs and Analysis of Their Pattern of Expression, P. J. McKinnon, S. K. McLaughlin, and R. F. Margolskee Cloning of Odorant Receptors, E. R. Liman and L. B. Buck List of Critical References for Techniques Not Covered

Experimental Cell Biology of Taste and Olfaction: Current Techniques and Protocols

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